MEGAscript® T7 Transcription Kit

Catalog number: AMB13345

Invitrogen™  Related applications: In Vitro Transcription

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Description

The MEGAscript® T7 Kit is an ultra-high yield in vitro transcription kit. The high yields are achieved by modifying typical transcription reaction conditions so that very high nucleotide concentrations can be effectively used. The SP6, T7, or T3 Enzyme Mix and the 10X Reaction Buffer are specifically calibrated for each lot and RNA polymerase.

Using MEGAscript® Kits
The MEGAscript® Kits contain in vitro transcription reaction components for twenty-five or forty 20 µL reactions and a control template. Each kit will yield a total of 3–5 mg of RNA (approximately 100 µg of RNA or more per reaction) from the control template supplied with the kit. This corresponds to 400–650 moles of RNA for each mole of template. Smaller templates typically yield a lower mass and a higher molar yield of product. Novel transcription reaction conditions and a patented, high-yield technology, allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions.

Applications
MEGAscript® Kits are intended for the synthesis of large amounts of unlabeled or low specific activity RNA for a variety of uses, including in vitro translation, antisense/microinjection studies, and isolation of RNA binding proteins. In large-scale transcription reactions, the concentration of all 4 nucleotides is high, well above the Km for the enzyme. MEGAscript® Kits typically yield over 10 times more RNA than conventional in vitro transcription reactions. MEGAscript® Kits are not recommended for synthesis of high specific activity probes.

Accessory products
MessageAmp™ aRNA Amplification Kits are powered by MEGAscript® technology and have been developed especially for the amplification of cRNA prior to array analysis. To purify in vitro transcription products from free nucleotides, buffer components, and enzymes, we recommend using the MEGAclear™ Kit (Cat. No. AM1908) and the MEGAclear™-96 Kit (Cat. No. AM1909).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Starting Material: Linearized DNA, PCR Products
Promoter: T7
RNA Yield: 100 µg
Final Product: In Vitro Transcribed RNA
Product Size: 200 reactions
Shipping Condition: Dry Ice

Contents & storage

T7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.

Documents

Manuals & protocols