Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: C10289

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function

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Description

The Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay provides a fast, sensitive, non-toxic and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy and high-content imaging. Click-iT® AHA is an amino acid analog of methionine containing an azido moiety. Similar to 35S-methionine, Click-iT® AHA is fed to cultured cells and incorporated into proteins during active protein synthesis. Detection of the incorporated amine acid utilizes a chemoselectiove ligation or “click" reaction between an azide and an alkyne, where the azido modified protein is detected with the green-fluorescent Alexa Fluor® 488 alkyne. The Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay has been successfully tested in A549 and U-2 OS cells with a variety of reagents that inhibit protein synthesis including cycloheximide, anisomycin and puromycin in both dose response and Min⁄Max format.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color: Blue, Green
Detection Method: Fluorescent
Emission Class: UV, Visible
Excitation Class: UV, Visible
For Use With (Equipment): Fluorescence Microscope, High Content Instrument
Format: 96-well plate
Label or Dye: Alexa Fluor™ 488, Hoechst 33258
Product Size: 2 plates
Green Features: Less hazardous
Shipping Condition: Room Temperature

Contents & storage

The kit contains sufficient reagents for performing 2 plates in a 96-well plate format.
Store at ≤-20°C, desiccate and protect from light.

Documents

Manuals & protocols