CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit

Catalog number: C10427

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Cellular Imaging

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Description

The CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. The kit includes the novel fluorogenic substrate CellEvent® Caspase-3/7 Green Detection Reagent, as well as SYTOX® AADvanced™ Dead Cell Stain.

• Caspase-3/7 specific—includes the recognition sequence for activated caspase-3 and caspase-7
• Easy identification—clearly identify live, dead, and apoptotic cell populations
• Quick analysis—washing and fixation is not required
• Multicolor compatibility—combine with other dyes excitable by the 488 nm laser or other lasers

View a selection guide for all apoptosis assays for flow cytometry

CellEvent® Caspase-3/7 Green Detection Reagent is a cell-permeant reagent that consists of a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye. During apoptosis, caspase-3 and caspase-7 proteins are activated and able to cleave the caspase 3/7 recognition sequence encoded in the DEVD peptide. Cleavage of the recognition sequence and binding of DNA by the reagent labels the apoptotic cells with a bright, fluorogenic signal that has absorption/emission maxima of ~511/533 nm. When used together with the SYTOX® AADvanced™ Dead Cell Stain, apoptotic cells can be easiliy discriminated from live and necrotic cells.

Because no single parameter defines apoptosis in all systems, we strongly suggest using a combination of different measurements for reliable detection of apoptosis. For more information, see Apoptosis Reagents in Flow Cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Tube(s)
Label or Dye: CellEvent™ Caspase 3/7 Green Detection Reagent, SYTOX® AADvanced dead cell stain
Product Size: 100 assays
Detection Method: Fluorescent
Number of Reactions: 100
For Use With (Equipment): Flow Cytometer
Excitation⁄Emission (nm): CellEvent™ Caspase 3/7 Green Detection Reagent: 511⁄533, SYTOX® AADvanced™: 546⁄ 647
Shipping Condition: Wet Ice

Contents & storage

Contains 1 vial CellEvent™ Caspase 3/7 Green Detection Reagent, 1 vial SYTOX® AADvanced dead cell stain, and 1 vial of DMSO.

Store all vials in the kit upright, at or below -20 °C, desiccated, and protected from light.

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