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Click-iT® LAA (linoleamide alkyne), for lipid peroxidation detection

Catalog number: C10447

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Cellular Imaging | Flow Cytometry | Labeling Chemistry

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Description

Click-iT® LAA (linoleamide alkyne) for lipid peroxidation detection, when combined with copper-catalyzed click chemistry, enables the detection of lipid-peroxidation-derived protein modifications in fixed cells. Click-iT® detection reagents are not included with this product. For a complete kit, please see our Click-iT® Lipid Peroxidation Imaging Kit - Alexa Fluor® 488.

How It Works
Click-iT® LAA is incubated with cells, where it incorporates into cellular membranes. Upon lipid peroxidation, LAA is oxidized and produces 9- and 13- hydroperoxy-octadecadienoic acid (HPODE). These hydroperoxides decompose to multiple α,β-unsaturated aldehydes, which readily modify proteins at nucleophilic side chains. These alkyne-containing modified proteins can be subsequently detected using Click-iT® chemistry and multiplexed with other probes appropriate for fixed cells. This assay is amenable to detection by traditional fluorescence microscopy, high content screening (HCS), and flow cytometry.

Available in Two Formats
Click-iT® LAA (linoleamide alkyne) is a stand-alone reagent provided as a five pack set of single-use vials for maximum flexibility in assays for lipid peroxidation. This reagent will need to be combined with appropriate azide-modified detection reagents and other reagents in the Click-iT® tool box. For a more complete kit, we offer the Click-iT® Lipid Peroxidation Imaging Kit - Alexa Fluor® 488, which includes the components you need to detect lipid peroxidation in cells, including a positive control compound.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Indicator Type: Lipid Peroxidation Sensor
Form: Frozen
Solubility: DMSO (Dimethylsulfoxide)
Product Size: 5 x 0.4 mg
Detection Method: Fluorescent, Chemiluminescent, Biotin-Based, Gel Electrophoresis, Colorimetric
For Use With (Equipment): Fluorescence Microscope, High Content Instrument, HTS Reader, Flow Cytometer, Floid® Cell Imaging System, Attune® Acoustic Focusing Cytometer
Sub-Cellular Localization: Cytoplasm
Shipping Condition: Wet Ice

Contents & storage

Store at ≤-20 °C
Protect from light
Desiccate when possible

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Manuals & protocols

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