CellROX® Orange Flow Cytometry Assay Kit

Catalog number: C10493

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Flow Cytometry

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Description

The CellROX™ Orange Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX™ Orange Reagent, as well as SYTOX™ Red Dead Cell Stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS).

View a selection guide for all CellROX® Reagents and Kits.

The CellROX™ Orange Flow Cytometry Assay Kit features:

• Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS
• Multicolor compatibility—minimal overlap with fluorophores excited by other laser lines, allowing easy multiplexing with other reagents
• Simple protocol—cells can be stained in complete media or other appropriate buffer, no need for serum free media

The CellROX™ Orange Detection Reagent is cell-permeable and non-fluorescent or very weakly fluorescent while in the reduced state. Upon oxidation, the reagent exhibits a strong fluorogenic signal that has absorption/emission maxima of 545/565 nm and remains localized in the cytoplasm. When used together with the included SYTOX™ Red Dead Cell Stain, oxidatively stressed and nonstressed cells are reliably distinguished from dead cells by flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Compatible Cells: Eukaryotic Cells, Mammalian Cells
For Use With (Equipment): Flow Cytometer
Detection Method: Fluorescent
Format: Tube(s)
Solubility: DMSO (Dimethylsulfoxide)
Label or Dye: CellROX® Orange Reagent
Product Size: 100 assays
Number of Reactions: 100
Flow Cytometer Laser Lines: 532, 633/635
Excitation⁄Emission (nm): SYTOX® red: 640⁄658, CellROX® orange: 545⁄565
Shipping Condition: Wet Ice

Contents & storage

Contains 1 vial CellROX® Orange Reagent (25 µL), 1 vial SYTOX® red dead cell stain (100 µL), 2 vials N-acetylcysteine (10 mg per vial), 1 vial tert-butyl hydroperoxide (50 µL, 70% solution in water), and 1 vial DMSO (200 µL).

Store kit at -5 to -30°C, protected from light. CellROX® reagent is air sensitive.

Documents

Manuals & protocols