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CellLight® Tubulin-GFP, BacMam 2.0

Catalog number: C10509

Molecular Probes™  Related applications: Cell Structure | Cell Viability, Proliferation & Function | Cellular Imaging

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Description

CellLight® Tubulin-GFP, BacMam 2.0, provides an easy method for the labeling of tubulin with Green Fluorescent Protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning. This ready-to-use construct is transduced/transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to human tubulin. You can observe tubulin-GFP behavior in live cells using a fluorescent imaging system and standard FITC/GFP filter set. Study dynamic action of tubulin filaments in live cells with almost no cytotoxicity. In addition, you can co-transduce/transfect more than one CellLight® reagent and label live cells with multiple tracking or tracing dyes to image dynamic cellular processes. Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexing with antibodies using immunocytochemical techniques.

CellLight® Technology
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose

Learn more about CellLight® fluorescent protein labeling

BacMam Technology
CellLight® Tubulin-GFP, BacMam 2.0, is a fusion construct of human tubulin and emGFP, providing accurate and specific targeting to cellular tubulin-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Liquid
Color: Green
Technique: Fluorescence Intensity, In Vivo Imaging
Label or Dye: GFP (EmGFP)
Product Size: 100 µL
Emission Class: Visible
Delivery Method: Baculovirus (BacMam System)
Detection Method: Fluorescent
Excitation Class: Visible
For Use With (Equipment): Fluorescence Microscope, Microscope, Floid® Cell Imaging System, Attune® Acoustic Focusing Cytometer, Confocal Microscope
Sub-Cellular Localization: Tubulin
Flow Cytometer Laser Lines: 488
Excitation⁄Emission (nm): 488⁄510
Shipping Condition: Wet Ice

Contents & storage

Store at 2-6° C, protected from light. Do Not Freeze.

Documents

Manuals & protocols