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CaptAvidin™ Agarose (Sedimented Bead Suspension)

Catalog number: C21386

Molecular Probes™  Related applications: Protein Assays and Analysis | Protein Purification & Isolation

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Description

CaptAvidin™ biotin-binding protein conjugated to agarose is designed for use in affinity chromatography. CaptAvidin™ biotin-binding protein contains a nitrated tyrosine in its biotin-binding site. This chemical modification permits dissociation of the avidin-biotin complex under milder conditions than the traditional avidin-biotin complex. At pH 4.0, CaptAvidin™ biotin-binding protein binds biotin tightly with a Ka of ~109M-1. This association can then be reversed at pH 10, allowing complete dissociation of the avidin-biotin complex. This is in contrast to the harsh conditions (8 M guanidine hydrochloride, pH 1.5) needed to dissociate the avidin-biotin complex.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: Agarose
Product Size: 5 mL
Shipping Condition: Wet Ice

Contents & storage

Store in refrigerator (2–8°C).

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