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Click-IT® GlcNAz Metabolic Glycoprotein Labeling Reagent (tetraacetylated N-Azidoacetylglucosamine) This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: C33367

Molecular Probes™  Related applications: Labeling Chemistry | Protein Gel Electrophoresis | Protein Labeling & Crosslinking | Protein Mass Spectrometry Analysis

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Description

The Click-iT® GlcNAz metabolic glycoprotein labeling reagent provides the first part of a simple and robust two-step technique to identify and characterize intracellular O-GlcNAc glycoproteins. In step one, cultured cells are incubated with the azide-modified glucosamine (GlcNAc). The azido-sugar is incorporated into intracellular O-GlcNAc-containing glycoproteins through the permissive nature of the oligosaccharide biosynthesis pathway. In step two, via the chemoselective ligation or click reaction between an azide and an alkyne, the azido-labeled glycoproteins can then detected with a Click-iT® Glycoprotein Detection kit for gels (TAMRA or Dapoxyl® alkyne) or Western blots (biotin alkyne). The Click-iT® glycoprotein products are compatible with LC-MS⁄MS and Multiplexed Proteomics™ technologies for in-depth analyses of the glycoproteome.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: TMR (Tetramethylrhodamine), Oregon Green® 488, Alexa Fluor™ 555, Alexa Fluor™ 594, Alexa Fluor™ 488, Biotin, Alexa Fluor™ 647
Product Size: 5.2 mg
Green Features: Less hazardous
Labeling Target: Proteins (Glycoproteins)
Detection Method: Fluorescent, Biotin-Based
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C).

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