Invitrogen™

MultiShot™ StripWell TOP10 Chemically Competent E. coli

Catalog number:  C409601

 Related applications: Transformation

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Description

MultiShot StripWell TOP10 chemically competent E. coli cells are cloning competent cells that packaged in a rack containing 12 strips of 8 tubes to increase productivity for medium-throughput bacterial transformations. TOP10 competent E. coli cells are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high-copy number plasmids and are the same competent cells that come with many of our cloning kits.

Versatile TOP10 cloning capabilities
MultiShot FlexPlate TOP10 competent E. coli cells are the laboratory workhorse for chemical transformations as they are compatible with a broad spectrum of DNA types for transformation. The TOP10 strain is useful for many cloning applications and provides transformation efficiencies in the MultiShot FlexPlate format of >1 x 108 cfu/µg with control plasmid DNA.

Key features of the MultiShot FlexPlate TOP10 Competent Cells include:
hsdR for efficient transformation of unmethylated DNA from PCR amplifications
mcrA for efficient transformation of methylated DNA from genomic preparations
lacZΔM15 for blue/white color screening of recombinant clones
endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I
recA1 for reduced occurrence of non-specific recombination in cloned DNA

MultiShot StripWell format—flexible for no waste
Each StripWell of eight connected tubes is designed with the same single-tube, single-use features as our One Shot format tubes. This format allows for all steps of the transformation protocol, up to plating, to take place in the same tube, thereby saving time and preventing contamination. After addition of DNA, MultiShot StripWell competent cells can be transformed by heat-shock at 42°C using a water bath, all in the same tube.

The StripWell tubes can be cut to permit transformations in a single tube, eliminating wasted aliquots of cells and avoiding freeze-thaws that can result in reduced transformation efficiency.

Key features of the MultiShot StripWell format include:
Increased productivity—for medium throughput experimentation
Maximal flexibility—transform as few as 1 tube and as many as 96 from the StripWell rack
Familiar convenience—no aliquotting, add DNA directly to cells, heat shock, and recover in the same tube
Peace of mind—single-use transformation minimizes contamination

Genotype: F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara, leu)7697 galU galK rpsL (StrR) endA1 nupG

Find the strain and format that you need
We offer many other E. coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot formatted comp cells or email us for custom formatting options.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Competent Cell Type: Chemically Competent
Species: E. coli
Format: MultiShot StripWell
Transformation Efficiency: > 1x10^8
Bacterial or Yeast Strain: TOP10
Product Line: MultiShot™
Blue-White Screening: Yes
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
F' Episome (to make ssDNA): Lacks F' Episome
High Throughput Compatibility: High Throughput-Compatible
Improves Plasmid Quality: Yes
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Size: 1 rack
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
T1 Phage - Resistant (tonA): No

Contents & storage

Contains:
• MultiShot™ StripWell TOP10 Chemically Competent E. coli: One 96-stripwell plate, 50 µl per well
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 2 bottles, 15 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

Documents

Manuals & protocols

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