One Shot® INV110 Chemically Competent E. coli

Catalog number: C717103

Invitrogen™  Related applications: Transformation

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Description

INV110 E. coliare derived from JM110 E. coliand are specifically designed for the growth and purification of plasmid DNA that will be digested with dam or dcm methylation-sensitive restriction enzymes. INV110 is a significant improvement over the JM110 strain. INV110 has been engineered to carry an endA1 mutation, which eliminates the non-specific endonuclease Endonuclease I, for improved plasmid DNA preparation. In addition, the INV110 genotype features:

• Blue/white screening of recombinant clones (lacZΔM15)
• Reduced cleavage of foreign methylated DNA for improved transformation of genomic DNA [Δ(mcrC-mrr)]
• F´ episome for phage infection and rescue of single-strand DNA
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Competent Cell Type: Chemically Competent
Species: E. coli
Format: One Shot
Transformation Efficiency: > 1x10^6
Bacterial or Yeast Strain: INV110
Product Size: 20 x 50 µL
Blue-White Screening: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Reduces Recombination: No
Cloning Methylated DNA: No
Improves Plasmid Quality: Yes
Propagating ccdB Vectors: Not for ccdB vector propagation
F' Episome (to make ssDNA): Contains F' Episome
Preparing Unmethylated DNA: Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA): No
High Throughput Compatibility: Not High Throughput-Compatible (Manual)

Contents & storage

One Shot® INV110 Chemically Competent E. coli contains 10 transformations of chemically competent cells in One Shot® format. Store at -80°C.

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Manuals & protocols

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