ChargeSwitch® gDNA Plant Kit This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: CS18000

Invitrogen™  Related applications: DNA Extraction

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Description

The ChargeSwitch® gDNA Plant Kit uses magnetic bead-based technology to allow rapid and efficient isolation of genomic DNA from fungal and plant samples, including leaves, tissues and seeds, in less than 15 minutes after lysate preparation.
• Optimized for higher-purity DNA extraction
• Compatible with high-throughput protocols
• Ideal for a variety of downstream applications

Rapid, Three Step, pH Based Purification Protocol
The ChargeSwitch® gDNA Plant Kit allows purification of genomic DNA from between 50-100 mg of fungi and plant samples, including leaves, tissues and seeds (Figure 1), to yield up to 7 µg of gDNA typically in less than 15 minutes following sample preparation and lysis. The ChargeSwitch® gDNA Plant Kit uses magnetic beads with a unique, ionizable functional group whose affinity for nucleic acids is pH-dependent to facilitate nucleic acid purification. Genomic DNA is isolated without the use of centrifugation or organic solvents, and is of higher purity than silica-prepared DNA, which is typically contaminated with guanidine salts.

Compatible with Single Tube and High-Throughput Protocols
The ChargeSwitch® gDNA Plant Kit is designed for automated processing of both small numbers of samples individually, as well as large numbers of samples using a liquid handling robot or a multi-channel pipette.

For processing up to 24 individual samples at a time the MagnaRack™ two-piece magnetic separation tube rack is required. This rack consists of a magnetic base station and a removable tube rack, which can hold up to 24 microcentrifuge tubes and can be used with most ChargeSwitch® products.

For high-throughput applications utilizing 96 well blocks, the 96-Well Magnetic Separator is required. This rack can hold and separate up to 96 magnetic bead samples in a deep well plate when placed on top of the magnetic base station.

Improved Performance in Demanding Downstream Applications
The ChargeSwitch® gDNA Plant Kit ensures reliable performance of the resulting gDNA and is routinely used for the most sensitive applications, including genetically modified organism (GMO) screening. Other downstream applications include PCR, restriction enzyme digestion, and Southern blotting.

For Research Use Only. Not intended for diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Fungi, Plant Samples
Isolation Technology: ChargeSwitch® Chemistry
Downstream Application: Cloning, Sequencing, Southern Blotting, PCR
High Throughput Compatibility: High Throughput-Compatible
Product Size: 96 preps
Green Features: Less hazardous, Less waste
Shipping Condition: Room Temperature

Contents & storage

All components of the ChargeSwitch® gDNA Plant Kit are shipped at room temperature. Upon receipt, store RNase A at 4“C. Store all other kit components at room temperature.

Components:

ChargeSwitch® Magnetic Beads (25 mg/ml in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
ChargeSwitch® Lysis Buffer (L18)
ChargeSwitch® Precipitation Buffer (N5)
ChargeSwitch® Wash Buffer (W12)
ChargeSwitch® Elution Buffer (E6; 10 mM Tris-HCl, pH 8.5 with 1 mM EDTA)
RNase A (5 mg/ml in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
10% SDS in deionized water
10% Detergent (D1)

Documents

Manuals & protocols