EnzChek® Phospholipase A1 Assay Kit

Catalog number: E10219

Molecular Probes™  Related applications: Protein Assays and Analysis

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Description

This kits takes our stand-alone assay for phospholipase A1, PLA1 (A10070) and combines the necessary reagents to run 2 to 10 complete 96 well microplate assays that monitor activity in purified enzyme preparations and cell lysates. The importance of phospholipases in cellular signaling, lipid metabolism, inflammatory responses and pathological disorders related to these processes has stimulated demand for fluorescence-based activity monitoring methods. In particular the phospholipases resident in plasma and endothelium can perturb circulating LDL and HDL particles, creating pro-artherogenic forms. Recent evidence is drawing a link between these lipases and the progression of several severe neurodegenerative diseases, including Alzheimer's. Molecular Probes’ fluorogenic phospholipase A1 substrates is designed to provide continuous monitoring of phospholipase A1 (PLA1) in purified enzyme preparations, cell lysates and living cells. PLA-1 improves upon two existing reagents in two ways: first it is now PLA-1 specific and secondly it has improved the assay quality by decreasing initial background noise.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Technique: Fluorescence Intensity
Assay Type: Phospholipase Assay
Product Size: 1 kit
Target Enzyme: Phospholipase
Substrate Type: Phospholipase Substrate
Number of Reactions: 200 Reactions
Substrate Properties: Chemical Substrate, Lipid-Based Substrate
Sample Type (Specific): Cell Extracts
Shipping Condition: Room Temperature

Contents & storage

1 kit. Store kit at -<20°C, protected from light, desiccated

Sufficient material is supplied for 200 reactions in 96 well microplates at 100 µl per well as described in the protocol below or 400 reaction in 384 low volume microplates at a  volume of ≤ 25 µL per well.

Documents

Manuals & protocols