EnzChek® Ultra Amylase Assay Kit

Catalog number: E33651

Molecular Probes™  Related applications: Cellular Imaging | Protein Assays and Analysis

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Description

The EnzChek® Ultra Amylase Assay Kit provides a solution-based assay featuring the speed, high sensitivity, and convenience required for measuring amylase activity or for screening amylase inhibitors in a high-throughput format. This EnzChek® kit contains a starch derivative—the DQ™ starch substrate—that is labeled with BODIPY® FL dye to such a degree that the fluorescence is quenched. This substrate is efficiently degraded by amylase; digestion relieves the quenching and yields highly fluorescent fragments. The accompanying increase in fluorescence is proportional to amylase activity and can be monitored with a fluorescence microplate reader or fluorometer, using standard fluorescein filters.

EnzChek® Ultra Amylase Assay Kit Specifications:
• Label (Ex/Em): BODIPY® FL conjugate (~502/512 nm)
• Kit contains lyophilized substrate, 10X reaction buffer, substrate solvent, a fluorescent standard, and a detailed protocol
• Sufficient reagents are supplied for 500 assays (using a 100 µL assay volume in a 96-well microplate assay format)


Find Fluorescent Substrates for Other Glycosidases
In addition to the EnzChek® Ultra Amylase Assay Kit, we offer kits and substrates to measure xylanase, lysozyme, β-galactosidase, and more. Review Detecting Glycosidases—Section 10.2 in the Molecular Probes® Handbook for more information on these products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Technique: Fluorescence Intensity
Assay Type: Amylase Assay
Product Size: 1 kit
Target Enzyme: Amylase
Substrate Type: Amylase Substrate
Substrate Properties: Molecular Substrate
Shipping Condition: Room Temperature

Contents & storage

Store at room temperature and protect from light.

Documents

Manuals & protocols