Fluo-4 Direct™ Calcium Assay Kit

Catalog number: F10471

Molecular Probes™  Related applications: Target & Lead Identification & Validation

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Description

The Fluo-4 Direct™ Calcium Assay Kit was formulated to provide a homogeneous fluorescent calcium assay that:
1. Easily loads into cells
2. Achieves a large assay window and
3. Suppresses background fluorescence generated from the calcium indicator in complete media with little-to-no impact on the specific cellular fluorescence

This advanced formulation allows the assay to be run in a simple “addition only" format in the presence of serum-containing media. Fluo-4 Direct™ is the third addition to the Fluo-4 family of calcium detection reagents. Fluo-4 AM and Fluo-4 NW both require media removal before assay detection, but Fluo-4 NW adds convenience by including the PowerLoad™ reagent for ease in cell loading. The Fluo-4 Direct™ Calcium Assay Kit is similar to Fluo-4 NW in that it is formulated with PowerLoad™ for ease in cell loading, but unique in that it can be used in the presence of complete culture media and will efficiently suppress background fluorescence without sacrificing the specific cellular fluorescence generated in the assay.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Indicator Type: Calcium Indicator
Indicator Properties: Fluorescent Dye-Based
For Use With (Equipment): Microplate Reader, Fluorescent Imager, Confocal Microscope, HTS Reader, High Content Instrument, Fluorescence Microscope
Cell Permeability: Cell-Permeant
Form: Powder
Color: Green
Product Size: 10 x 10 mL
Emission Class: Visible
Volume (Metric): 100 ml
Detection Method: Fluorescent
Excitation Class: Visible
Sub-Cellular Localization: Nucleus, Organelles, Cytoplasm
Excitation⁄Emission (nm): 488⁄530
Shipping Condition: Room Temperature

Contents & storage

• 10 × 10 ml Fluo-4 Direct™ Calcium Assay Reagent (Component A)
• 2 × 77 mg Probenecid (Component B)
• 200 ml Fluo-4 Direct™ Calcium Assay Buffer (Component C)

Store at ≤-20°C. Dessicate and protect from light.

Documents

Manuals & protocols

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