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FISH Tag™ RNA Multicolor Kit, Alexa Fluor® dye combination

Catalog number: F32956

Molecular Probes™  Related applications: Cellular Imaging | Labeling Chemistry | Nucleic Acid Labeling & Oligo Synthesis

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Description

The FISH Tag™ RNA Multicolor Kit provides a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. The FISH Tag™ RNA Multicolor Kit contains fluorescent dyes in four colors, making it ideal for the simultaneous localization of multiple sequence-specific RNA species in cells, tissues, and whole embryo mounts. We also offer FISH Tag™ Kits in single colors.

FISH Tag™ Kit Specifications:

• Dye (Ex/Em): Alexa Fluor® 488 (492/520 nm), Alexa Fluor® 555 (555/565 nm), Alexa Fluor® 594 (590/615 nm), Alexa Fluor® 647 (650/670 nm)
• Comprehensive kit includes 16 reagents
• Comes with detailed protocol to help ensure your success


Complete Workflow Solution for FISH
FISH Tag™ Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor® dyes. PureLink® nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade® Gold antifade reagent is added to preserve the fluorescent signal during imaging.

More Options for Nucleic Acid Labeling
To review various options for nucleic acid labeling (including DNA and RNA FISH), review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes® Handbook. For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: Alexa Fluor™ 647, Alexa Fluor™ 488, Alexa Fluor™ 594, Alexa Fluor™ 555
Product Size: 1 kit
Target Molecule: RNA
Detection Method: Fluorescent
Target Specificity: Not Target-Specific
Shipping Condition: Dry Ice

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.

Documents

Manuals & protocols