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HCS Mitochondrial Health Kit

Catalog number: H10295

Molecular Probes™  Related applications: Cell Structure | Cell Viability, Proliferation & Function

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Description

The HCS Mitochondrial Health Kit simultaneously and quantitatively measures two important cell-health parameters: mitotoxicity and cytotoxicity. The MitoHealth stain detects changes in the mitochondrial membrane potential, whereupon the reagent accumulates in active mitochondria. The kit includes the Image-iT® DEAD™ Green to detect cytotoxicity, an impermeant dye to healthy cells that becomes permeant when the plasma membrane of cells is compromised. Both indicators are amenable to fixation and permeabilization, thus allowing for multiplexing with other biomarkers of interest. The blue-fluorescent nuclear segmentation tool Hoechst 33342 is also included and stains DNA in both live and dead cells.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color: Blue, Green, Orange
Detection Method: Fluorescent
Emission Class: UV, Visible
Excitation Class: UV, Visible
Excitation⁄Emission (nm): 350⁄461, 488⁄515, 550⁄580
For Use With (Equipment): Fluorescence Microscope, High Content Instrument
Format: 96-well plate
Label or Dye: Hoechst 33342, Image-iT® DEAD Green viability stain, MitoHealth stain
Product Size: 1 kit
Shipping Condition: Room Temperature

Contents & storage

The kit contains sufficient reagents for performing 2 plates in a 96-well plate format.
Store at ≤-20°C, desiccate and protect from light.

Documents

Manuals & protocols