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Image-iT® LIVE Green Poly Caspases Detection Kit, for microscopy

Catalog number: I35104

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Cellular Imaging | Target-Based ADME⁄Tox Assays

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Description

The Image-iT™ LIVE Green Poly Caspases Detection Kit employs a novel approach to detect active caspases that is based on a fluorescent inhibitor of caspases (FLICA™) methodology, essentially an affinity label. The reagent associates a fluoromethyl ketone (FMK) moiety, which can react covalently with a cysteine, with a caspase-specific amine acid sequence. For poly caspases, this recognition sequence is valine-alanine-aspartic acid (VAD). A fluorescein group is attached as a reporter. The FLICA reagent is thought to interact with the enzymatic reactive center of an activated caspase via the recognition sequence, and then to attach covalently through the FMK moiety. The FLICA inhibitor is cell permeant and noncytotoxic. Unbound FLICA molecules diffuse out of the cell and are washed away; the remaining green-fluorescent signal is a direct measure of the amount of active caspase that was present at the time the inhibitor was added.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Slide(s)
Label or Dye: Propidium iodide, Hoechst 33342, FAM™
Product Size: 1 kit
Detection Method: Fluorescent
For Use With (Equipment): Fluorescence Microscope
Shipping Condition: Room Temperature

Contents & storage

Store in refrigerator (2–8°C) and protect from light.

Documents

Manuals & protocols

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