iPrep™ PureLink® gDNA Blood Kit

Catalog number: IS10005

Invitrogen™  Related applications: Automated Nucleic Acid Purification | DNA Extraction

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Description

The iPrep™ PureLink® gDNA Blood Kit allows rapid and automated extraction of genomic DNA (gDNA) from human blood including fresh or frozen blood samples, and concentrated leukocyte preparations (buffy coat). Genomic DNA is extracted from blood samples using the Dynabeads® MyOne™ SILANE and iPrep™ Purification Instrument within 30 minutes without the use of centrifugation. The purified genomic DNA is suitable for use in downstream applications, including PCR. Advantages of the iPrep™ PureLink® gDNA Blood Kit:

• Obtain high yields of gDNA from up to 350 µL blood
• Isolate highly pure gDNA with high A260/A280 and A260/A230 ratios
• Experience superior performance in sensitive downstream applications
• Purify high-quality gDNA from fresh or frozen whole blood

iPrep™ Purification Instrument
The iPrep™ PureLink® gDNA Blood Kit is designed for use with the iPrep™ Purification Instrument. The iPrep™ Purification Instrument is a benchtop, automated nucleic acid purification instrument with integrated Magnetic and Syringe Unit capable of purifying nucleic acids from up to 13 samples (12 samples + 1 positive control) using magnetic bead-based technology.

Using the iPrep™ PureLink® gDNA Blood Kit
With A260/A280 ratios of 1.8, the resulting DNA is highly pure (see figure), ideal for use in sensitive multiplex assays (see table) or for long-term storage. This kit is optimized to isolate highly-pure DNA from 150 µL to 350 µL of fresh or frozen blood in less than 30 minutes (see figure). It is highly tolerant of anticoagulants such as citrate and EDTA, and yields high molecular weight DNA. The yield is highly reproducible (see figure), and there is no cross-contamination between samples (see figure). Furthermore, the kit can also be used with cultured cells. The table summarizes the performance of the iPrep™ PureLink® gDNA Blood Kit. The iPrep™ PureLink® gDNA Blood Kit comes with all reagents (supplied in prefilled cartridges), tubes, and tips needed to isolate DNA from blood samples. All you need to do is add the cartridges, tips, and your sample tubes to the instrument, select your preferred elution volume, and come back 30 minutes later to collect your pure gDNA in individual screwcap elution tubes.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Blood
Isolation Technology: Magnetic Bead
Downstream Application: PCR, Sequencing, Southern Blotting
Final Product: Genomic DNA
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Product Size: 1 kit
Shipping Condition: Room Temperature
Starting Material (Amount): 150 µl to 350 µl blood samples

Contents & storage

The iPrep™ PureLink® gDNA Blood Kit contains enough material to perform 52 purifications

Contains:
• 1 iPrep™ PureLink® gDNA Blood Cartridge Kit
• 2 × 52 iPrep™ Sample and Elution Tubes
• 1 bag iPrep™ Tips and Tip Holders (52 tips and holders)

Store at room temperature. Do not freeze the cartridge kit.

Documents

Manuals & protocols

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