LyticBLAzer™-FRET B/G Homogeneous Kit

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Description

LyticBLAzer™ -FRET B/G Substrate Kits provides a ratiometric, end-point method of analyzing beta-lactamase activity in cell lysates. The LyticBLAzer™ -FRET B/G Reagent combines the components for lysis and detection in a single reagent addition, resulting in a very simple assay. Use the LyticBLAzer™ on top-read or bottom-read instrumentation. LyticBLAzer™ Substrate Kits are available in homogeneous as well as non-homogeneous formats (Figure 1), each optimized for a different assay condition.

LyticBLAzer™ FRET B/G Substrate Kit Advantages:

• Ratiometric blue:green reporter readout reduces false hits by minimizing experimental noise
• Integrated lysis and detection components enable use with top-read or bottom read fluorescence instrumentation
• Endpoint analysis and stable fluorescence yield longer read times

LyticBLAzer™ -BODIPY® FL Substrate Kit:
LyticBLAzer™ -BODIPY® FL Substrate Kits provides an end-point, non-ratiometric method of analyzing beta-lactamase activity in cell lysates. The LyticBLAzer™ -BODIPY® FL Reagent combines the components for lysis and detection in a single reagent addition, resulting in a very simple assay. Use the LyticBLAzer™ -BODIPY® FL Assays on top-read or bottom-read instrumentation. LyticBLAzer™ -BODIPY® FL Substrate Kits are available in homogeneous as well as non-homogeneous formats (Figure 1), each optimized for a different assay condition.

LyticBLAzer™ -BODIPY® FL Substrate Kit Advantages:
• BODIPY® fluorophore excitation avoids fluorescence interference caused by sample contaminants such as dust, lint, etc.
• Standard excitation and emission wavelengths are universally adaptable to all fluorescence instrumentation.
• Integrated lysis and detection components enable use with top-read or bottom read fluorescence instrumentation.
• Endpoint analysis and stable fluorescence yield longer read times
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Substrate: LyticBLAzer™-FRET B/G
Product Size: 3.75 L
Substrate Type: Beta-Lactamase Substrate
Detection Method: Fluorescent
Substrate Properties: Chemical Substrate
For Use With (Equipment): Microplate Reader, Fluorometer

Contents & storage

LyticBLAzer™ homogenous kits: LyticBLAzer™-FRET B/G Homogenous Loading Kits include LyticBLAzer™_h-FRET B/G Substrate, DMSO (for Solution A), and Homogenous Lysis Buffer. LyticBLAzer™_h-FRET B/G Substrate (provided lyophilized) should be stored at -20°C, and protected from light. LyticBLAzer™_h-FRET B/G Substrate should also be dessicated. DMSO (for Solution A) and Homogenous Lysis Buffer should be stored at 22-25°C, protected from direct light.

The 150 ml kit is sufficient for 3,750 x 40 µl standard protocol well assays; the 3.75 L kit is sufficient for 93,750 x 40 µl standard protocol well assays. Bulk quantities of loading kits are available upon request.

LyticBLAzer™ standard kits: LyticBLAzer™-FRET B/G Loading Kits include LyticBLAzer™-FRET B/G substrate, DMSO (for Solution A), and Lysis Buffer. LyticBLAzer™-FRET B/G substrate is provided lyophilized and should be stored at -20“C, dessicated and protected from light. DMSO (for Solution A) and Lysis Buffer should be stored at 22-25°C, protected from direct light.

The 150 ml kit is sufficient for 3,750 x 40 µl standard protocol well assays; the 3.75 L kit is sufficient for 93,750 x 40 µl standard protocol well assays. Bulk quantities of loading kits are available upon request.

LyticBLAzer™_h-BODIPY® FL Substrate, DMSO (for Solution A), and Homogenous Lysis Buffer. LyticBLAzer™_h-BODIPY® FL Substrate (provided lyophilized) should be stored at -20“C, and protected from light. LyticBLAzer™_h-BODIPY® FL Substrate should also be
dessicated. DMSO (for Solution A) and Homogenous Lysis Buffer should be stored at 22-25°C, protected from direct light.

LyticBLAzer™-BODIPY® FL Loading Kits include LyticBLAzer™-BODIPY® FL substrate, DMSO (for Solution A), and Lysis Buffer. LyticBLAzer™-BODIPY® FL substrate is provided lyophilized and should be stored at -20°C, dessicated and protected from light. DMSO (for Solution A) and Lysis Buffer should be stored at 22-25°C, protected from direct light.

Documents

Manuals & protocols