GeneBLAzer® VDR DA Assay Kit

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Description

GeneBLAzer®VDR DA(Division Arrested) cells and VDR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Vitamin D receptor (VDR) fused to the DNAbinding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla HEK 293T cell line. GeneBLAzer®UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-VDR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both VDR DA cells and VDR-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of Calcitriol (1α, 25-dihydroxyvitamin D3). In addition, VDR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

System: GeneBLAzer®
Gene ID: 7421
Reporter: BLA (Beta-Lactamase)
Cell Line: 293T (HEK)
Cell State: Division-Arrested Cells
Gene Symbol: VDR
Product Size: 1 plate
Primary Agonist: TO901317
Detection Method: Fluorescent
Druggable Target: Nuclear Receptors

Contents & storage

Shipping: Dry Ice Storage: -80°C/Liquid Nitrogen Sufficient division-arrested cells and substrate to assay one 384-well plate. Includes: VDR HEK 293T DA cells (K1417A) LiveBLAzer™-FRET B/G Loading Kit, 70 μg

Documents

Manuals & protocols