β-Gal Assay Kit

Catalog number: K145501

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

The β-Gal Assay Kit provides the reagents required to quickly measure the levels of active β-galactosidase expressed in cells transfected with plasmids expressing the lacZ gene. lacZ is a bacterial gene often used as a reporter construct in eukaryotic transfection experiments. The gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates and its activity is easily assayed. β-galactosidase catalyzes the hydrolysis of β-galactosidase such as ortho-nitrophenyl-D-galactopyranoside (ONPG). Hydrolysis of ONPG to the ONP anion produces a bright yellow color with a peak absorbance at 420 nm that can be quantified using a spectrophotometer.
10.7 gene expression
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Substrate: ONPG
Assay Type: β-Gal (lacZ) Assay
Product Size: 80 mL
Target Enzyme: Beta-Galactosidase
Substrate Type: Beta-Gal Substrate
Detection Method: Colorimetric
Substrate Properties: Chemical Substrate

Contents & storage

The β-Gal Assay Kit contains 10X PBS (phosphate buffered saline), lysis buffer, cleavage buffer, β-mercaptoethanol, ONPG, stop buffer. Store ONPG at -20°C. All other components are stored at either room temperature or +4°C. All reagents are guaranteed stable for 6 months when properly stored.

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