mRNA Catcher™ PLUS Purification Kit

      Catalog number: K157002

      Invitrogen™  Related applications: Automated Nucleic Acid Purification | RNA Extraction

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      Description

      The mRNA Catcher™ PLUS Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format. By taking advantage of Locked Nucleic Acid (LNA) technology, the mRNA Catcher™ PLUS Kit provides improved performance and a faster protocol, while preserving mRNA yield and purity (see table). The mRNA Catcher™ PLUS Kit offers:

      • Robust performance—high binding capacity per well (>80 ng/well)
      • Reproducible results—low CVs from well-to-well and plate-to-plate (~2%)
      • Optimized protocols—for small sample sizes (100 cells, 4 mg tissue, and 40 µL blood)
      • Compatible plate design—may be used with most PCR cyclers

      How it works
      mRNA from total RNA, cells, tissue, and blood samples is hybridized to an immobilized, single-stranded 20-mer oligo(dT)-containing LNA. The incorporation of LNA into the oligo probe increases the hybridization efficiency and specificity of the mRNA binding. The mRNA can either be eluted or directly reversed-transcribed to cDNA directly in the well. The mRNA Catcher™ PLUS method does not require centrifugation, precipitation, phase separation, or column filtration. In addition, the entire method is amenable to automation for streamlined, high-throughput expression profiling for large-scale processes. Isolated mRNA is suitable for use in downstream applications such as RT-PCR and qRT-PCR.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Sample Type (General): Blood, Cells, RNA, Tissue
      High Throughput Compatibility: Automated Protocols, High Throughput-Compatible
      Downstream Application: Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction
      Elution Volume - max: 80 µl
      Elution Volume - min: 80 µl
      Final Product: mRNA
      For Use With (Equipment): Robotic Liquid Handling Systems
      Format: 96-well plate
      Isolation Technology: Oligo dT Affinity
      Number of Reactions: 96 Preps
      Product Line: mRNA CATCHER™
      Product Size: 96 preps
      Purification Time: 1.5 hrs
      Quantity: 1
      Sample Type (Specific): Mammalian Cells, Tissue (Mammalian), Total RNA, Whole Blood
      System: mRNA Catcher
      Shipping Condition: Wet Ice

      Contents & storage

      • 1 96-well mRNA Catcher™ PLUS plate (4°C)
      • 4 ml 2X Lysis Buffer L20 (room temperature)
      • 30 ml Wash Buffer W15 (room temperature)
      • 4 ml Dilution Buffer DL1 (room temperature)
      • 8 ml Elution Buffer E3 (room temperature)
      • 100 µl 0.5M DTT (4°C)

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