mRNA Catcher™ PLUS Purification Kit

Description

The mRNA Catcher™ PLUS Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format. By taking advantage of Locked Nucleic Acid (LNA) technology, the mRNA Catcher™ PLUS Kit provides improved performance and a faster protocol, while preserving mRNA yield and purity (see table). The mRNA Catcher™ PLUS Kit offers:

• Robust performance—high binding capacity per well (>80 ng/well)
• Reproducible results—low CVs from well-to-well and plate-to-plate (~2%)
• Optimized protocols—for small sample sizes (100 cells, 4 mg tissue, and 40 µL blood)
• Compatible plate design—may be used with most PCR cyclers

How it works
mRNA from total RNA, cells, tissue, and blood samples is hybridized to an immobilized, single-stranded 20-mer oligo(dT)-containing LNA. The incorporation of LNA into the oligo probe increases the hybridization efficiency and specificity of the mRNA binding. The mRNA can either be eluted or directly reversed-transcribed to cDNA directly in the well. The mRNA Catcher™ PLUS method does not require centrifugation, precipitation, phase separation, or column filtration. In addition, the entire method is amenable to automation for streamlined, high-throughput expression profiling for large-scale processes. Isolated mRNA is suitable for use in downstream applications such as RT-PCR and qRT-PCR.

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Specifications

Sample Type (General):	Blood, Cells, RNA, Tissue
High Throughput Compatibility:	Automated Protocols, High Throughput-Compatible
Downstream Application:	Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction
Elution Volume - max:	80 µl
Elution Volume - min:	80 µl
Final Product:	mRNA
For Use With (Equipment):	Robotic Liquid Handling Systems
Format:	96-well plate
Isolation Technology:	Oligo dT Affinity
Number of Reactions:	96 Preps
Product Line:	mRNA CATCHER™
Product Size:	96 preps
Purification Time:	1.5 hrs
Quantity:	1
Sample Type (Specific):	Mammalian Cells, Tissue (Mammalian), Total RNA, Whole Blood
System:	mRNA Catcher
Shipping Condition:	Wet Ice

Contents & storage