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GeneBLAzer® TRα-UAS-bla HEK 293T Assay Kit

Catalog number: K1684

 Related applications: Target & Lead Identification & Validation

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Description

TR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Thyroid hormone receptor alpha(TR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla HEK 293T cell line. GeneBLAzer®UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-TR alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. TR alpha-UASbla HEK 293T 293 cells are functionally validated for Z' and EC50 concentrations of T3 Thryoid hormone. In addition, TR alpha-UAS-bla HEK 293T 293 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

System: GeneBLAzer®
Reporter: BLA (Beta-Lactamase)
Cell State: Dividing Cells
Antagonists: AGN 193109
Product Size: 1 plate
Primary Agonist: All-trans-retinoic Acid
Detection Method: Fluorescent
Druggable Target: Nuclear Receptors
Alternate Agonists: 9-cis-retinoic acid, AM 580, CD437, TTNPB

Contents & storage

Shipping: Dry Ice Storage: -80°C/Liquid Nitrogen Contains: TR alpha-UAS-bla HEK 293T cells (K1260)

Documents

Manuals & protocols