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GeneBLAzer® PPARγ-UAS-bla HEK 293H Cells

Catalog number: K1701

 Related applications: Pharma & Biopharma | Target & Lead Identification & Validation

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Description

PPAR gamma-UAS-bla HEK 293H cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-gamma (PPAR gamma) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla HEK 293H cell line. GeneBLAzer®UAS-bla HEK 293H cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-PPAR gamma (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. PPAR gamma-UAS-bla HEK 293H cells are functionally validated for Z' and EC50 concentrations of Rosiglitazone. In addition, PPAR gamma-UAS-bla HEK 293H cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell State: Dividing Cells
Detection Method: Fluorescent
Druggable Target: Nuclear Receptors
Primary Agonist: Calcitriol
Product Size: 2 x 106 cells
Reporter: BLA (Beta-Lactamase)
System: GeneBLAzer®

Contents & storage

Shipping: Dry Ice Storage: -80°C/Liquid Nitrogen Contains: PPAR gamma-UAS-bla 293H cells (K1094)

Documents

Manuals & protocols