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CellSensor® Gli-bla 22Rv.1 Cell Line

Catalog number: K1838

 Related applications: Target & Lead Identification & Validation

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Description

The morphogenic signal Shh provides in the developing CNS induces proliferation of neuronal precursor cells in the developing cerebellum and other tissues. Proliferative signaling by Shh is involved in the development of cancer, including specific brain and skin cancers such as basal cell carcinomas. Signaling takes place through a Patched (PTC-1)⁄Smoothened (SMO) Receptor complex. The activation of Patched by Shh releases the inhibition of Patched on Smoothened leading to Sonic Hedgehog pathway activation of the Gli transcription factor to induce downstream gene expression. The CellSensor® Gli-bla 22Rv1 cell line contains a beta-lactamase reporter gene under control of the Gli response element stably integrated into 22Rv1 cells. This cell line is a clonal population isolated by flow cytometry. The clone was selected based on inhibition of the pathway with KAAD-Cyclopamine. This cell line has also been tested for assay performance under variable conditions, including DMSO concentration, cell number, compound incubation time, and substrate loading time and validated for Z’ under maximal inhibition of β-lactamase activity with Clavulanic Acid.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

System: CellSensor™
Readout: End Point
Reporter: BLA (Beta-Lactamase)
Technique: FRET
Cell State: Dividing Cells
Antagonists: KAAD-Cyclopamine
Gene Symbol: GLI
Product Size: 1 vial
Primary Agonist: Constitutive
Detection Method: Fluorescent
Druggable Target: Kinases, Signaling Pathway

Contents & storage

CellSensor® Gli-bla 22Rv.1 cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.

Documents

Manuals & protocols