PureLink® HiPure Plasmid Midiprep Kit

Catalog number: K210005

Invitrogen™  Related applications: DNA Extraction

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Description

The PureLink® HiPure Plasmid Midiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Midiprep Kit protocol will typically yield 100–350 µg plasmid DNA from 15–25 mL bacterial culture, at a purity that is comparable to that achieved by two passes through cesium chloride gradients. Advantages of the PureLink® HiPure Plasmid Midiprep Kit include:

Labor and time saving—no need for extra steps to remove endotoxin and other contaminants
High-purity, low-endotoxin product—pure enough for mammalian cell transfection
Versatility—purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs

Anion-exchange chromatography for plasmid purification
The PureLink™ HiPure Plasmid DNA Purification Kits use a patented anion-exchange resin to purify plasmid DNA to a level equivalent to two passes through CsCl gradients. The resin combines excellent capacity with a fast flow rate, high resolution, high yield, and efficient endotoxin removal. Plasmid preparations can typically be completed in less than 2 hours.

Contaminant-free DNA
Unlike cesium chloride gradient protocols, PureLink® HiPure Plasmid Purification Systems do not use organic solvents, ethidium bromide, or cesium chloride, all of which are troublesome to work with and to dispose of. Typically, plasmid DNA prepared using the PureLink® HiPure Plasmid Midiprep Kit will have an A260/A280 ratio of >1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications. Endotoxin levels are typically within 0.1–1 EU/µg, making this plasmid DNA ideal for mammalian cell transfection.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Sample Type (General): Bacteria
Downstream Application: PCR, Transfection, In Vitro Transcription, Next-Generation Sequencing, Sequencing, Nucleic Acid Labeling, Transformation, Cloning
System: PureLink™
Quantity: 50 columns
Plasmid Type: <40kb, BAC, Low Copy Plasmid, High Copy Plasmid
Product Size: 50 preps
Final Product: Plasmid DNA, BAC DNA
Yield (up to): 350 µg
Purification Time: 2 hrs
Number of Reactions: 50 Preps
Elution Volume - max: 5 ml
Elution Volume - min: 5 ml
Isolation Technology: Anion Exchange Resin with Lysate Clearing Filter
Sample Type (Specific): Bacterial Culture
Purity or Quality Grade: Transfection Grade
Shipping Condition: Room Temperature

Contents & storage

• 200 ml Resuspension Buffer (R3)
• 1.5 ml RNase A
• 200 ml Lysis Buffer (L7)
• 200 ml Precipitation Buffer (N3)
• 2 × 250 ml Equilibration Buffer (EQ1)
• 2 × 500 ml Wash Buffer (W8)
• 250 ml Elution Buffer (E4)
• 30 ml TE Buffer
• 50 HiPure Columns
• 10 Column Holders

Store all components at room temperature.

Documents

Manuals & protocols

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