PureLink® Quick Plasmid Miniprep Kit

Catalog number: K210010

Invitrogen™  Related applications: DNA Extraction

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Description

The PureLink® Quick Plasmid Miniprep Kit is designed for rapid isolation of sequencing-grade plasmid DNA from E.coli in about 30 minutes, using silica membrane columns. Features of the PureLink® Quick Plasmid Miniprep Kit:

Fast—ready-to-use plasmid DNA typically in about 30 min
Flexible—formats work with vacuum and centrifugation platforms

Reliable and cost-effective isolation of plasmid DNA
The PureLink® Quick Plasmid Miniprep Kit allows isolation of high-quality plasmid DNA from 1–5 mL cultures in 30–45 min, using the PureLink® spin columns. These columns use a unique silica membrane to achieve high yields of up to 40 µg of sequencing-grade plasmid DNA. Cells are lysed using an alkaline/SDS procedure and the lysate is applied to a silica membrane column that selectively binds plasmid DNA. Contaminants are removed with Wash Buffer, and the plasmid DNA is eluted in TE Buffer. The eluted DNA is suitable for all routine downstream applications.

Compatible with flexible formats
The PureLink® Quick Plasmid Miniprep Kit can be used with a centrifuge or a vacuum manifold for purifying plasmid DNA. Typically, DNA isolated using the PureLink® Quick Plasmid Miniprep Kit has an A260/A280 of <1.80 when samples are diluted in Tris-HCl (pH 7.5), indicating that the DNA is reasonably free of proteins that could interfere with downstream applications.

Isolated DNA can be used for a variety of downstream applications
Plasmid DNA purified using PureLink® Quick Plasmid Miniprep Kit is suitable for all routine downstream applications, including bacterial and mammalian cell transformations, DNA sequencing, restriction enzyme digestion, cloning, and PCR.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Sample Type (General): Bacteria
Downstream Application: PCR, Sequencing, Cloning, Transformation, Nucleic Acid Labeling, Next-Generation Sequencing
Product Size: 50 preps
Final Product: Plasmid DNA
Isolation Technology: Silica Spin Column
Sample Type (Specific): Bacterial Culture
Shipping Condition: Room Temperature

Contents & storage

All components of the PureLink® Quick Plasmid DNA Miniprep Kits are shipped at room temperature. Upon receipt, store all components at room temperature.

Components:
Resuspension Buffer (R3)
RNase A (20 mg/ml)
Lysis Buffer (L7)
Precipitation Buffer (N4)
Wash Buffer (W9)
Wash Buffer (W10)
TE Buffer (TE)
Spin Columns
Wash and Recovery Tubes

Documents

Manuals & protocols

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