TOPO® TA Cloning® Kit, Dual Promoter, with One Shot® TOP10 chemically competent E. coli cells

Catalog number: K460040

Invitrogen™  Related applications: PCR Cloning

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Description

The TOPO® TA Cloning® Dual Promoter Kit is for fast, efficient cloning and subsequent in vitro transcription/translation. This kit includes the pCR™II-TOPO® TA vector (see figure). By eliminating time-consuming and tedious restriction site cloning, TOPO® cloning is the most reliable method, taking only 5 minutes using a 3-step protocol, and yielding up to 95% recombinants. Convenient features of the TOPO® TA Cloning® Dual Promoter Kit include:

• 3´-T overhangs for direct ligation of Taq-amplified PCR products
• T7 and SP6 promoters for efficient in vitro transcription
• M13 forward and reverse primer sites for sequencing
• 16 convenient restriction sites, including EcoRI, flanking your insert for subsequent excision or subcloning
• Kanamycin and ampicillin resistance for your choice of selection in E. coli
• Easy blue/white colony screening for selection of recombinants
• Includes OneShot® TOP10 Competent cells for added convenience and highest cloning efficiencies
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Vector Type: TOPO-TA Cloning Vectors
Cloning Method: TOPO®-TA
Bacterial or Yeast Strain: TOP10
Product Size: 50 reactions

Contents & storage

Box 1:
• Topoisomerase I-activated pCR™II-TOPO® vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot® Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid.

Store at -68 to -85°C.

Documents

Manuals & protocols

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