Champion™ pET302/NT-His and pET303/CT-His Vector Kit

      Numéro de catalogue:  K630203

      Invitrogen™  Applications liées: Protein Expression

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      Description

      The Champion™ pET302/NT-His and pET303/CT-His Vector Kit is designed for cloning a gene of interest via restriction enzyme(s) and ligase (REaL) and subsequent high-level expression from the strong bacteriophage T7 promoter. In addition to the T7 promoter, each vector contains only the necessary functional elements and an N- or C-terminal 6xHis tag (pET302/NT-His and pET303/CT-His, respectively) for convenient purification and detection (Figure 1). Expression levels obtained from these vectors may be higher than those obtained from another suppliers pET vector (Figure 2). To maximize expression, use with MagicMedia™ E. coli Expression Medium.


      Contents and Storage:

      The Champion™ pET302/NT-His and pET303/CT-His Vector Kit includes 6 µg each of pET302/NT-His and pET303/CT-His and 10 µg of a control vector. Store at -20“C. All components are guaranteed stable for 6 months when properly stored.
      For Research Use Only. Not for use in diagnostic procedures.

      Spécifications

      Vector Type: pDEST, pET
      Cloning Method: Restriction Enzyme ⁄ MCS
      Promoter: T7
      Protein Tag or Fusion: His Tag (6x)
      Antibiotic Resistance (Bacterial): Ampicillin (AmpR)
      Constitutive or Inducible System: Inducible
      Inducing Agent: IPTG
      Product Line: Champion™
      Product Size: 10 µg
      Selection Agent (Eukaryotic): None

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