SAA Livestock ELISA Kit

Catalog number: KAA0021

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

The SAA Multispecies ELISA Kit quantifies Serum Amyloid A (SAA) in bovine, porcine, canine, feline, or equine serum and plasma samples. Serum Amyloid A is an acute phase protein produced in the liver and may increase significantly following inflammation, infection, or injury. This convenient kit helps save you time by utilizing a simple protocol and a pre-coated 96-well plate. The SAA Multispecies ELISA Kit is manufactured by Tridelta Development Limited.

The SAA Multispecies ELISA Kit includes all necessary reagents to run the assay:
• SAA Antibody-Coated Wells, 1 × 96 well plate
• Wash Buffer Concentrate, 1 × 50 mL
• Sample/Calibrator Diluent Concentrate, 1 × 25 mL
• SAA Calibrator, 1 lyophilized vial
• Anti-SAA-HRP Conjugate, 1 × 6 mL
• Stabilized Chromogen, Tetramethylbenzidine (TMB), 1 × 11 mL
• Stop Reagent, 1 × 11 mL
• Detailed Protocol, 1 protocol

Your Results Ready Typically in Less Than 2 Hours
ELISA kits are fast and easy-to-use. Add your diluted serum or plasma sample, standards, and controls to the pre-coated 96-well plate and incubate for 1 hour. After incubation, perform a single wash step, incubate for 15 minutes with substrate, add stop solution, and your plate is ready to be read (Figure 1).

For the SAA Multispecies ELISA Kit, we recommend using a serum dilution of at least 1:500 for most samples (use 1:2000 for equine samples).

Part of the Invitrogen™ ELISA Kit Family
Invitrogen™ has a wide menu of ELISA kits targeted to disease-related proteins. With world class-leading sensitivity, proven specificity and consistent performance, these optimized ELISA kits offer you a choice when it comes to getting physiologically relevant and quantitative results.

Learn more about Invitrogen™ ELISA Kits
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Incubation Time: 1.25 hr
Assay Range: 0.062-0.5 µg/mL
Assay Sensitivity: <0.5 pg/mL
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Novex
Gene Aliases: PIG4, SAA, SAA2, TP53I4
Gene ID: 6288
Gene Name: serum amyloid A1
Gene Symbol: SAA1
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Aliases: SAA, Amyloid fibril protein AA
Protein Name: Serum amyloid A-1 protein
Sample Type (Specific): Plasma, Serum
Sample Volume: 50 µL
Shipping Condition: Wet Ice
Target (Kit Named): SAA
Target Species (Named): Livestock
Target Species (Validated): Bovine, Canine, Equine, Feline, Porcine
UniProt ID: P0DJI8

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody(s), diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols