Insulin Human ELISA Kit

Catalog number:  KAQ1251

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

The Human Insulin ELISA research-use-only kit is to be used for the quantitative determination of human insulin in serum samples using 96-well plates and a microplate reader.

Performance characteristics
• Sensitivity: 0.17 µIU/mL
• Standard curve range: 5.1‒250 µIU/mL
• Sample types: serum
• Species cross-reactivity: human (bovine, canine, porcine, rabbit)
• Sample volume: 50 μL
• Total assay incubation time: 0.75 hrs

Principle of the method
The Human Insulin ELISA kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA). The assay uses monoclonal antibodies (mAbs) directed against distinct epitopes of insulin. Samples including standards of known insulin content, control specimens, and unknowns are pipetted into the wells. A detector monoclonal antibody labeled with horseradish peroxidase (HRP) is added. After an incubation period, the microtiter plate is washed to remove unbound enzyme-labeled antibody and a substrate solution (tetramethylbenzidine [TMB]–H202) is added and incubated. The reaction is stopped with HCl and the microtiter plate is read spectrophotometrically. The intensity of color is directly proportional to the concentration of insulin in the original specimen.

Background information
Insulin, a polypeptide hormone with a molecular weight of 5,800 Da, is secreted by the beta cells of pancreatic Islets of Langerhans. Insulin possesses a wide spectrum of biological actions. It stimulates cellular glucose uptake, glucose oxidation, glycogenesis, lipogenesis, proteogenesis, and the formation of DNA and RNA. Insulin plays a key role in the regulation of plasma glucose levels (hepatic output inhibition, stimulation of peripheral glucose utilization). The resulting hypoglycemic effects of insulin are counterbalanced by hormones with hyperglycemic effects (glucagon, growth hormone, cortisol, epinephrine). Insulin secretion is controlled by the plasma glucose levels: hyperglycemia induces a prompt increase in circulating insulin levels. Neural influences, as well as various metabolic and hormonal factors (amino acids, glucagon, gastrointestinal hormone) also participate in the control of insulin secretion.

Each ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency. See product insert for more information on validation.

Related links
Learn more about ELISA kits
Learn more about other immunoassays
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: Hormones & Receptors
Assay Incubation Time: 0.75 hr
Assay Range: 5.1-250 µlU/mL
Assay Sensitivity: 0.17 µlU/mL
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Novex
Gene Aliases: IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10
Gene ID: 3630
Gene Name: insulin
Gene Symbol: INS
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Name: Insulin
Sample Type (Specific): Plasma, Serum
Sample Volume: 50 µL
Shipping Condition: Wet Ice
Target (Kit Named): Insulin
Target Species (Named): Human
Target Species (Validated): Human
UniProt ID: P01308

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody(s), diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols

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