Amyloid beta 40 Human ELISA Kit

Catalog number: KHB3481

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

The Human Beta Amyloid [1-40] (Aβ40) ELISA research-use-only kit is to be used for the quantitative determination of human Aβ40 in samples (e.g., cell culture supernatants, tissue homogenates, cerebrospinal fluid [CSF], etc.) using 96-well plates and a microplate reader. The assay recognizes both natural and synthetic forms of human Aβ40. The anti-human Aβ40 antibody used in this kit is capable of selectively detecting Aβ40 and not Aβ42/Aβ43.*

Performance characteristics
• Sensitivity: <6 pg/mL
• Standard curve range: 7.81–500 pg/mL
• Sample types: cell culture supernatants, tissue homogenates, cerebrospinal fluid (CSF)
• Species cross-reactivity: human
• Sample volume: 50 μL (prediluted)
• Total assay incubation time: 4 hrs

Principle of the method
The Human Aβ40 kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA). A monoclonal antibody specific for the NH2-terminus of human Aβ has been coated onto the wells of the microtiter strips provided. During the first incubation, standards of known human Aβ40 content, controls, and unknown samples are pipetted into the wells and co-incubated with a rabbit antibody specific for the COOH-terminus of the 1-40 Aβ sequence. This COOH-terminal sequence is created upon cleavage of the analyzed precursor.

After washing, bound rabbit antibody is detected by the addition of a horseradish peroxidase–labeled anti-rabbit antibody. After a second incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of human Aβ40 present in the original specimen.

Background information
Beta amyloid peptide (Aβ) is a 40 to 43 amino acid peptide cleaved from amyloid precursor protein by β-secretase (e.g., BACE) and a putative γ (gamma) secretase.* Increased release of the longer forms of Aβ peptide, Aβ42 or Aβ43, which have a greater tendency to aggregate than Aβ40, occurs in individuals expressing certain genetic mutations, expressing certain ApoE alleles, or may involve other, still undiscovered, factors. Many researchers theorize that it is this increased release of Aβ42/Aβ43 which leads to the abnormal deposition of Aβ.*

Each Human Aβ40 ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency. See product insert for more information on validation.

Related links
Learn more about ELISA kits
Learn more about other immunoassays

*Please refer to the ELISA protocol for specific reference citations.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Incubation Time: 4 hr
Assay Range: 9.8-500 pg/mL
Assay Sensitivity: <6 pg/mL
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Novex
Gene Aliases: AAA, ABETA, ABPP, AD1I, CTFgamma, CVAP, PN-II, PN2, APP
Gene ID: 351
Gene Name: amyloid beta precursor protein
Gene Symbol: APP
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Name: Amyloid beta A4 protein
Sample Type (Specific): Cerebrospinal Fluid, Tissue Homogenates
Sample Volume: 50 µL
Shipping Condition: Wet Ice
Target (Kit Named): Amyloid beta 40
Target Species (Named): Human
Target Species (Validated): Human
UniProt ID: P05067

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols

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