TNF alpha ELISA Kit, Human

Catalog number: KHC3012

Novex™  Related applications: Protein Assays and Analysis

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Description

The Human Tumor Necrosis Factor alpha (TNF alpha, TNF) ELISA research-use-only kit is to be used for the quantitative determination of TNF alpha in samples (see sample types indicated) using 96-well plates and a microplate reader. The assay will recognize both natural and recombinant forms of this target.

Performance characteristics

• Sensitivity: <1.7 pg/mL
• Standard curve range: 15.6‒1,000 pg/mL
• Sample type(s): serum, plasma, cell culture supernatant
• Specificity: natural and recombinant human TNF alpha
• Cross-reactivity: see kit manual for cross-species and/or cross-target reactivity
• Sample volume: 100 μL
• Total assay time: 4 hours

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Principle of the method

The human TNF alpha solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Target information

Human Tumor Necrosis Factor-Alpha (TNF-α), also called cachectin, is a 157 AA nonglycosylated polypeptide cytokine mainly produced by activated macrophages. Lipopolysaccharide (LPS) is a potent stimulus for TNF-α production in macrophages. TNF-α is an important mediator of the in vivo effects of LPS. The various biological activities of TNF-α may be classified as:

- Immunomodulating and proinflammatory activities: TNF-α regulates the production of antibodies by B cells and stimulates cytotoxic T cells.

- Metabolic activities: TNF-α strongly inhibits lipoprotein lipase and adipocyte gene expression.

Related links

Learn more about ELISA kits
Learn more about other immunoassays

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gene Aliases: DIF, TNF-alpha, TNFA, TNFSF2
Accession Number: NP_000585.2, NM_000594.2
Shipping Condition: Wet Ice
Marker: TNF-α
Gene ID: 7124
Species: Human
Gene Symbol: TNF
Sensitivity: <1.7 pg⁄mL
Target Gene: tumor necrosis factor
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 192 assays
Concentration: 15.6-1000 pg⁄ml
Sample Volume: 100 µl
Protein Family: Cytokines & Receptors
Incubation Time: 4 hrs
Detection Method: Colorimetric
Research Category: Immunology, Apoptosis
Sample Type (Specific): Plasma, Supernatants, Serum
For Use With (Equipment): Microplate Reader

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols

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