NF kappaB p65 (total) ELISA Kit, Human

Catalog number: KHO0371

Novex™  Related applications: Protein Assays and Analysis

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Description

The NF-κBp65 (Total) ELISA kit is designed to detect and quantify the level of NF-κBp65 protein. This assay is intended for the detection of NF-κBp65 from human and mouse cell lysates using 96-well plates and a microplate reader.

Easy to run
More sensitive than Westerns
Convenient precoated, removable 8-well strips

Easy-to-Run Sandwich ELISA
A monoclonal capture antibody specific for NF-κBp65 (regardless of phosphorylation site) has been coated onto the wells of the 96-well plate provided. Samples, including a standard of known NF-κBp65 (Total) content, controls, and unknowns, are pipetted into these wells and then a rabbit detection antibody specific for NF-κBp65 (Total) is added to the wells. During the first incubation, the NF-κBp65 antigen binds to the immobilized capture antibody and the NF-κBp65 rabbit antibody serves as a detection antibody by binding to the immobilized NF-κBp65 (Total) protein. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of NF-κBp65 (Total) present in the original specimen and the optical density can be read on a standard microplate reader. The total assay incubation time is only 4 hours.

Detect Low Expressing Proteins and Use Less Sample
ELISA kits designed to measure intracellular signaling targets are 2–10X more sensitive than Western blotting. The improved sensitivity enables you to detect low-expressing proteins that otherwise may not be distinguishable from background. In addition, the amount of sample needed to run the assay is less than what is needed for Western blots.

Ready-to-Use NF-κBp65 Total ELISA Kit Contains These Reagents, Buffers, and Supplies:

• NF-κBp65 Antibody-Coated 96-Well Plate
• NF-κBp65 Total Standard
• Standard Diluent Buffer
• NF-κBp65 Total Detection Antibody
• anti-rabbit IgG-HRP (100X)
• HRP Diluent
• Wash Buffer Concentrate (25X)
• Stabilized Chromogen, TMB
• Stop Solution
• Plate Covers
• Detailed protocol with validation tests

Convenient Kit Format
The kit comes with a capture antibody precoated to the plate, eliminating an overnight coating process. The plates are machine coated to provide low variability well-to-well. Furthermore, the plate comes as removable 8-well strips allowing you to run as few samples as you want. In addition, the standards provided in the kit can be used to obtain quantitative results from the standard curve and/or can be used as a positive control.

Rigorous Validation
Each ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency. See protocol insert for more information on validation.

Part of the ELISA Kit Family
We have a wide array of ELISA kits targeted to disease-related proteins. With excellent sensitivity, proven specificity and consistent performance, these optimized ELISA kits offer you a choice when it comes to getting physiologically relevant and quantitative results.

Learn more about ELISA kits.
Learn more about other immunoassays.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gene Aliases: NF-kappaB, NFKB-p105, NFKB-p50, NFkappaB, p105, p50, DKFZp686C01211, EBP-1, KBF1, MGC54151, NF-kappaB1, NF-kappa-B
Accession Number: NM_001165412.1, NM_003998.3, NP_003989.2, NP_001158884.1
Shipping Condition: Wet Ice
Marker: NF-κBp65
Gene ID: 4790
Species: Mouse, Human
Gene Symbol: NFKB1
Sensitivity: 50 pg/mL
Target Gene: nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 assays
Concentration: 78-5000 pg⁄ml
Sample Volume: 10 µl
Protein Family: Transcription Factors
Incubation Time: 4 hrs
Detection Method: Colorimetric
Research Category: Signal Transduction
Sample Type (Specific): Cell Extracts
For Use With (Equipment): Microplate Reader

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols