PicoPure® DNA Extraction Kit

Catalog number: KIT0103

Applied Biosystems™  Related applications: DNA Extraction | Laser Capture Microdissection

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Description

The ARCTURUS® PicoPure® DNA Extraction Kit offers an easy, streamlined genomic extraction procedure that produces PCR-ready DNA. The streamlined process enables reliable and reproducible DNA recovery from as few as ten cells.

Key product features:
• Streamlined workflow—simplify your genomic extraction for PCR-ready DNA
• Efficient extraction—maximize DNA recovery
• Flexible application—extract DNA for any PCR application
• Maximize compatibility—use with most tissue and cell preparation procedures

Go Direct to PCR
The PicoPure® DNA Extraction Kit offers an easy, streamlined genomic extraction procedure that produces PCR-ready DNA. Extract and amplify DNA in the same tube, without organic extraction or spin columns. Recover DNA from as few as ten cells procured by Laser Capture Microdissection (LCM) or from milligrams of tissue. The PicoPure® DNA Extraction Kit provides conveniently packaged, stable Proteinase K, PCR-compatible DNA Reconstitution Buffer and a complete user guide. Each of ten lyophilized Proteinase K aliquots can be freshly reconstituted to make 150 µl of PicoPure® DNA Extraction Solution-enough for 150 x 10 µl CapSure® HS LCM Cap extractions or 30 x 50 µl CapSure® Macro LCM Cap extractions (See Figure 1).

Maximize DNA Recovery
Because DNA is not lost due to organic extractions or spin columns, the PicoPure® Kit enables reliable and reproducible DNA recovery from as few as ten cells. PCR studies illustrate the advantages of the PicoPure® Kit. Equal amounts of DNA were transferred to PCR, either directly in the PicoPure Kit Buffer or after purification using a DNA Extraction Kit from a leading manufacturer. Quantitative Real-time PCR (QRT-PCR) demonstrates that the PicoPure® Kit samples amplify earlier, suggesting more DNA present, and amplify more consistently, indicating less sample variation, than column-purified samples. The PicoPure Kit's reliably high DNA recovery enables increased detection sensitivity, for example, when amplifying single-copy genes from small samples in mutation analysis or genotyping assays. This high DNA recovery makes the Kit ideal for small samples, such as those prepared by LCM (See Figure 2).

Extract DNA for Any PCR Application
DNA extracted with the PicoPure Kit is compatible with endpoint PCR-performed in the same 0.5 ml tube used for DNA extraction. For gene copy number quantitation, DNA from very small samples can be directly amplified by qRT-PCR without further purification, using platforms such as the StepOnePlus™ Real-time PCR System. For highly sensitive mutation and genotype analysis, DNA can be extracted and directly amplified from small samples, and gene fragments can be separated by dHPLC (denaturing High-Performance Liquid Chromatography), using platforms such as the WAVE® System (Transgenomics). (See Figures 3 & 4)

Compatible with Most Tissue and Cell Preparation Procedures
The PicoPure® DNA Extraction Kit enables successful recovery of genomic DNA from animal tissue sections and cell samples prepared using a wide range of methods*. Superior results are obtained from formalin-fixed paraffin-embedded (FFPE) tissue sections, frozen tissue sections, ethanol fixed cells, and cytological smears. (See Figure 5.)

*Not recommended for use with whole blood, plant or fungal samples.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): FFPE & Fixed Samples, Cells, Tissue
Sample Type (Specific): Tissue (FFPE), Tissue (Frozen), Laser Capture Microdissection (LCM)
Isolation Technology: Enzymatic
Downstream Application: PCR, Real-Time Quantitative PCR (qPCR)
Final Product: Genomic DNA
For Use With (Equipment): ArcturusXT™ LCM Instrument
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Format: Tube(s)
Product Size: 150 preps
Starting Material (Amount): As few as 10 cells.

Contents & storage

Lyophilized Proteinase K, Reconstitution Buffer. Store at room temperature.

Documents

Manuals & protocols