Arcturus® Paradise® PLUS qRT-PCR Kit

Catalog number: KIT0300

Applied Biosystems™  Related applications: Laser Capture Microdissection

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Description

The ARCTURUS® Paradise® PLUS Reagent System enables unprecedented gene expression analysis of millions of previously inaccessible samples. The Paradise® PLUS Reagent System is an integrated solution for gene expression studies from formalin-fixed, paraffin-embedded (FFPE) tissues, including reagents for tissue staining, RNA isolation and RNA amplification.

Key product features:
• Accelerated discovery process – unlock the tissue archives to access previously inaccessible samples
• Streamlined application – integrate seamlessly into current workflow
• Efficient workflow – secure downstream success with the Paradise® QC Kit
• Comprehensive system – benefit from the only complete system for formalin-fixed samples

Unlock the Tissue Archives
Currently, tens of millions of tissue samples with known outcomes are available in tissue archives. The Paradise® PLUS Reagent system enables microarray and qRT-PCR analysis of these previously inaccessible samples, allowing retrospective studies to accelerate discoveries. Achieve unprecedented results in just six steps (Figure 1).

Integrate Seamlessly into Current Workflow
The Paradise® PLUS Reagent System is compatible with all major gene expression platforms. Robust and reproducible results are achieved using tools and techniques commonly employed in today’s research laboratories.

Secure Downstream Success with the Paradise® QC Kit
Not all FFPE samples contain high quality RNA. Identify the best samples for your studies using a simple, real-time PCR research technique, and then proceed with confidence using these pre-qualified samples.

Benefit from the Only Complete System for Formalin-fixed Samples
The Paradise® PLUS Reagent System is the only available solution that provides all the components necessary to prepare RNA from formalin-fixed, paraffin-embedded tissue samples for gene expression analysis. To ensure successful gene expression results, the system includes reagents optimized for exceptional recovery of RNA and superior reverse transcription and RNA amplification (Figure 3).

Integrated Systems for Microgenomics
The Paradise® PLUS Reagent System is validated as part of Arcturus' complete Systems for Microgenomics®, an integrated solution for utilizing small quantities of RNA for gene expression analysis, and features the ARCTURUSXT Laser Capture Microdissection (LCM) Instrument to procure pure cell populations. ARCTURUS®' Systems for Microgenomics enable accurate and sensitive microarray assays that reveal molecules otherwise obscured in whole tissue samples or cell mixtures.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Size: 48 samples
Workflow Step: Reverse Transcription, RNA Extraction, Tissue Staining, RNA Amplification, In Vitro Transcription
Sample Type (General): FFPE & Fixed Samples
Downstream Application: Real-Time Quantitative PCR (qPCR)
Sample Type (Specific): Tissue (FFPE)
For Use With (Equipment): ArcturusXT™ LCM Instrument

Contents & storage

Paradise Staining Kit (stain, staining jars, dehydration reagents, LCM slides), RNA Isolation Kit (RNA extraction and purification reagents, purification columns), RNA Amplification (cDNA synthesis and IVT modules), Amplification Purification Kit (purification reagents and columns). Variable storage conditions.

Documents

Manuals & protocols