Arcturus® Paradise® PLUS 2 Round Kit, biotin labeling, no solvents

Catalog number: KIT0302BNS

Applied Biosystems™  Related applications: Laser Capture Microdissection

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Description

The ARCTURUS® Paradise® PLUS Reagent System with Microarray Labeling permits unprecedented gene expression analysis of millions of previously inaccessible samples. The Paradise® PLUS Reagent System is an integrated solution for gene expression studies from formalin-fixed, paraffin-embedded (FFPE) tissues, including reagents for tissue staining, RNA isolation and RNA amplification. Amplify RNA from as little as 5 ng of fixed RNA using methods that have been specially optimized to overcome the challenges of cross-linked templates.

Key product features:
• Microarray Labeling, Gene Expression Profiling
• Flexibility in Assay Format
• Integrated Systems for Microgenomics

Microarray Labeling, Gene Expression Profiling
The Paradise® PLUS RNA Amplification Kit may be configured to include Turbo Labeling™ for use in microarray experiments. Turbo Labeling™ Kits offer a proprietary, non-enzymatic technology optimized for the labeling of unmodified, amplified RNA (aRNA) for gene expression profiling. The unmodified aRNA is labeled post-amplification, thereby avoiding the need to incorporate modified nucleotides during RNA amplification. The use of unmodified nucleotides in the amplification process results in unmodified aRNA with higher yields and longer aRNA fragments, thus providing better representation of the mRNA transcript for downstream analysis.

Flexibility in Assay Format
Turbo Labeling™ Kits include reagents to support labeling of 12 samples using either Cy™3⁄Cy™5 dyes or Biotin for hybridization to cDNA or oligonucleotide arrays.

Integrated Systems for Microgenomics
The Paradise® PLUS Reagent System is validated as part of the ARCTURUS® Complete System for Microgenomics®, an integrated solution for utilizing small quantities of RNA for gene expression analysis, and features the ARCTURUSXT™ Laser Capture Microdissection (LCM) Instrument to procure pure cell populations. ARCTURUS®' Systems for Microgenomics enable accurate and sensitive microarray assays that reveal molecular otherwise obscured in whole tissue samples or cell mixtures.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Size: 48 samples
Workflow Step: Reverse Transcription, RNA Extraction, Nucleic Acid Labeling, Tissue Staining, RNA Amplification, In Vitro Transcription
Sample Type (General): FFPE & Fixed Samples
Downstream Application: Microarray Analysis
Sample Type (Specific): Tissue (FFPE)
For Use With (Equipment): ArcturusXT™ LCM Instrument

Contents & storage

Paradise Staining Kit (stain, staining jars, LCM slides), RNA Isolation Kit (RNA extraction and purification reagents, purification columns), RNA Amplification (cDNA synthesis and IVT modules), Amplification Purification Kit (purification reagents and columns),Turbo Biotin labeling kit (biotin labeling reagent, labeling buffer, purification columns). Variable storage conditions.

Documents

Manuals & protocols