Arcturus® Paradise® Plus staining components

Catalog number: KIT0312S

Applied Biosystems™  Related applications: Laser Capture Microdissection

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Description

The ARCTURUS® Paradise® PLUS FFPE LCM Staining Kit offers a simple solution developed to stain formalin-fixed paraffin-embedded (FFPE) tissues before proceeding with laser capture microdissection (LCM) and downstream molecular analysis. FFPE samples introduce unique challenges for gene expression profiling, including chemical modification and fragmentation of RNA molecules. Archival of FFPE samples adds to these challenges through increased RNA degradation over time.

Key product features:
• Provide Superior Staining while Preserving RNA
• Secure Downstream Success with the Paradise® QC Kit
• Benefit from the Only Complete System for Formalin-fixed Samples

Provide Superior Staining while Preserving RNA
The Paradise® PLUS FFPE LCM Staining Kit offers a simple solution developed to stain FFPE tissues before proceeding with laser microdissection and downstream molecular analysis. It is a fast penetrating stain that provides good contrast by differential staining of nuclei (purple) and cytoplasm (light pink). By minimizing the exposure of tissues to water where nucleases may be activated, the Paradise® PLUS FFPE LCM Staining Kit helps to preserve RNA integrity that may be otherwise compromised when using longer staining protocols.

Secure Downstream Success with the Paradise® QC Kit
Identify the best samples for your studies using the Paradise® PLUS Sample Quality Assessment Kit (QC Kit). The QC Kit uses a simple, real-time PCR technique to check the quality of RNA in your FFPE sample before staining and LCM. Proceed with confidence knowing your FFPE samples have high quality RNA.

Benefit from the Only Complete System for Formalin-fixed Samples
The Paradise® PLUS Reagent System is the only available solution that provides all the components necessary to prepare RNA from FFPE tissue samples for gene expression analysis. To ensure successful microarray results, the system includes reagents optimized for exceptional recovery of RNA and superior RNA amplification.

Integrated Systems for Microgenomics
The Paradise® PLUS Reagent System is validated as part of the ARCTURUS® System for Microgenomics, an integrated solution for utilizing small quantities of RNA for gene expression analysis. ARCTURUS® Systems for Microgenomics enable accurate and sensitive microarray assays that reveal molecular details otherwise obscured in whole tissue samples or cell mixtures.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Size: 24 samples
Workflow Step: Tissue Staining
Sample Type (General): FFPE & Fixed Samples
Downstream Application: Laser Capture Microdissection (LCM)
Sample Type (Specific): Tissue (FFPE)
For Use With (Equipment): ArcturusXT™ LCM Instrument

Contents & storage

Paradise Staining Kit (stain, solvents, staining jars and LCM slides). Store at room temperature.

Documents

Manuals & protocols