SAA ELISA Kit, Mouse

Catalog number: KMA0021

Novex™  Related applications: Protein Assays and Analysis

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Description

The Mouse Serum Amyloid A (SAA) ELISA research-use-only kit is to be used for the quantitative determination of mouse SAA in serum or plasma samples using 96-well plates and a microplate reader.

Performance characteristics
• Sensitivity: 0.03 μg/mL
• Standard curve range: 0.062–0.5 μg/mL
• Sample types: serum, plasma
• Species cross-reactivity: mouse
• Sample volume: 50 μL (1:200)
• Total assay incubation time: 1.25 hrs

Principle of the method
The Mouse SAA kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA). A monoclonal antibody specific for mouse SAA has been coated onto the wells of the microtiter strips provided. Mouse samples, including calibrators of known SAA content, are pipetted into micro wells together with an HRP-labeled anti-SAA antibody and incubated at 37°C. Any mouse SAA present will be captured between the coated microplate and the labeled antibody. The plate is washed manually after sample and antibody-HRP incubation to remove any unbound material. Following the addition of a substrate solution, a blue product is generated in direct proportion to the amount of mouse SAA present in the original sample or calibrator. The reaction is stopped with the addition of stop reagent.

Background information
Serum Amyloid A (SAA) is a low molecular weight acute phase protein produced in the liver in response to a series of stimuli such as viral or bacterial infection, trauma, or other causes of inflammation and tissue damage. During an acute phase response, the concentration of SAA in blood rises dramatically up to 1,000-fold excess above normal circulating levels. SAA has been demonstrated to have utility in predicting renal allograft rejections and in assessing the inflammatory status of arthritis. SAA has also been used to monitor recovery from infections and inflammation, with the levels falling rapidly in response to therapy.

Each Mouse SAA ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency. See product insert for more information on validation.

Related links
Learn more about ELISA kits
Learn more about other immunoassays
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Shipping Condition: Wet Ice
Concentration: 0.062-0.5 µg⁄ml
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Gene ID: 111345
Gene Symbol: SAA
Incubation Time: 1.25 hrs
Label or Dye: HRP (Horseradish Peroxidase)
Marker: SAA
Product Size: 96 assays
Research Category: Immunology, Inflammation
Sample Type (Specific): Plasma, Serum
Sample Volume: 50 µl
Sensitivity: 0.03 ug⁄ml
Species: Mouse
Target Gene: serum amyloid A cluster

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody(s), diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols