Amyloid beta 40 ELISA Kit, Mouse

Catalog number: KMB3481

Novex™  Related applications: Protein Assays and Analysis

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Description

The Mouse Beta Amyloid 1-40 (Aβ40) ELISA research use only kit is to be used for the quantitative determination of Mouse and Rat Aβ40 in samples (e.g., cell culture supernatants, tissue homogenates, cerebrospinal fluid [CSF], etc.) using 96-well plates and a microplate reader. The assay will recognize both natural and synthetic forms of Mouse and Rat Aβ40. The anti-mouse Aβ40 antibody used in this kit is capable of selectively detecting Aβ40 and not Aβ42 or Aβ43.

Performance characteristics
• Sensitivity: <5 pg/mL
• Standard curve range: 7.81–500 pg/mL
• Sample types: cell culture supernatants, tissue homogenates, cerebrospinal fluid (CSF)
• Species cross-reactivity: mouse, rat
• Sample volume: 100 μL (prediluted)
• Total assay incubation time: 4 hrs

Principle of the method
The Mouse Aβ40 kit is a solid phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA). A monoclonal antibody to the NH2-terminus of Mouse Aβ has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Mouse Aβ40 content, control specimens and unknowns, are pipetted into these wells. The Aβ antigen binds to the immobilized (capture) antibody. After washing, a rabbit monoclonal antibody specific for the COOH-terminus of the 1-40 Aβ sequence is added to the wells. Bound rabbit antibody is detected by the use of a horseradish peroxidase-labeled anti-rabbit antibody.

After removal of excess anti-rabbit antibody, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Mouse Aβ40 present in the original specimen.

Background information
Alzheimer’s Disease (AD) is characterized by the presence of extracellular plaques and intracellular neurofibrillary tangles (NFTs) in the brain. The major protein component of these plaques is beta amyloid peptide (Aβ), a 40 to 43 amino acid peptide cleaved from amyloid precursor protein by β-secretase (e.g., BACE) and a putative γ (gamma) secretase. Increased release of the ‘longer forms’ of Aβ peptide, Aβ42 or Aβ43, which have a greater tendency to aggregate than Aβ40, occurs in individuals expressing certain genetic mutations, expressing certain ApoE alleles, or may involve other, still undiscovered, factors. Many researchers theorize that it is this increased release of Aβ42/Aβ43 which leads to the abnormal deposition of Aβ and the associated neurotoxicity in the brains of affected individuals.

Each Mouse Aβ40 ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency. See product insert for more information on validation.

Related links
Learn more about ELISA kits
Learn more about other immunoassays

*Please refer to the ELISA protocol for specific reference citations.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gene Aliases: Abeta, Abpp, Adap, Ag, Cvap, E030013M08Rik, betaApp
Shipping Condition: Wet Ice
Marker: Aβ 40
Gene ID: 11820, 54226
Species: Mouse, Rat
Gene Symbol: App
Sensitivity: <5 pg⁄mL
Target Gene: amyloid beta (A4) precursor protein
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 assays
Concentration: 7.81-500 pg⁄ml
Sample Volume: 100 µl
Incubation Time: 4 hrs
Detection Method: Colorimetric
Research Category: Neurobiology
Sample Type (Specific): Supernatants, Cerebrospinal Fluid, Tissue Homogenates
For Use With (Equipment): Microplate Reader

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols

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