Tau (Total) Mouse ELISA Kit

Catalog number: KMB7011

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

The TAU (Total) Mouse ELISA kit is designed to detect and quantify the level of TAU protein. This assay is intended for the detection of TAU from mouse tissue homogenates and supernatants using 96-well plates and a microplate reader.

Features include:

• Simplicity—easy to run
• Sensitivity—greater than that of Western blotting
• Convenience—assays are performed in precoated, removable 8-well strips

Easy-to-Run Sandwich ELISA
A monoclonal capture antibody specific for TAU (regardless of phosphorylation state) has been coated onto the wells of the 96-well plate provided. Samples, including a standard of known TAU (Total) content, controls, and unknowns, are pipetted into these wells and then a rabbit detection antibody specific for TAU (Total) is added to the wells. During the first incubation, the TAU antigen binds to the immobilized capture antibody and the TAU rabbit antibody serves as a detection antibody by binding to the immobilized TAU (Total) protein. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of TAU (Total) present in the original specimen and the optical density can be read on a standard microplate reader. The total assay incubation time is only 4 hours.

Detect Low Expressing Proteins and Use Less Sample
ELISA kits designed to measure intracellular signaling targets are 2–10X more sensitive than Western blotting. The improved sensitivity enables you to detect low-expressing proteins that otherwise may not be distinguishable from background. In addition, the amount of sample needed to run the assay is less than what is needed for Western blots.

Convenient Kit Format
The kit comes with a capture antibody precoated to the plate, eliminating an overnight coating process. The plates are machine coated to provide low variability well-to-well. Furthermore, the plate comes as removable 8-well strips allowing you to run as few samples as you want. In addition, the standards provided in the kit can be used to obtain quantitative results from the standard curve and/or can be used as a positive control.

Rigorous Validation
Each ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency. See protocol insert for more information on validation.

Part of the ELISA Kit Family
We have a wide array of ELISA kits targeted to disease-related proteins. With excellent sensitivity, proven specificity and consistent performance, these optimized ELISA kits offer you a choice when it comes to getting physiologically relevant and quantitative results.

Learn more about ELISA kits.
Learn more about other immunoassays.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Incubation Time: 4 hr
Assay Range: 31-2000 pg/mL
Assay Sensitivity: <15 pg/mL
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Novex
Gene Aliases: AI413597, AW045860, Mtapt, Tau
Gene ID: 17762
Gene Name: microtubule-associated protein tau
Gene Symbol: MAPT
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Aliases: Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Protein Name: Microtubule-associated protein tau
Sample Type (Specific): Cell Culture Supernatants, Tissue Homogenates
Sample Volume: 50 µL
Shipping Condition: Wet Ice
Target (Kit Named): Tau
Target Species (Named): Mouse
Target Species (Validated): Mouse
Target Sub-Specificity: Total
UniProt ID: P10637

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols

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