IL-2 Mouse ELISA Kit

Catalog number:  KMC0021C

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

The Mouse Interleukin-2 (IL-2, IL2) ELISA research-use-only kit is to be used for the quantitative determination of IL-2 in samples (see sample types indicated) using 96-well plates and a microplate reader. The assay will recognize both natural and recombinant forms of this target.

Performance characteristics

• Sensitivity: <4 pg/mL
• Standard curve range: 15.6–1,000 pg/mL
• Sample type(s): serum, plasma, cell culture supernatant
• Specificity: natural and recombinant mouse IL-2
• Cross-reactivity: see kit manual for cross-species and/or cross-target reactivity
• Sample volume: 50 μL
• Total assay time: 3 hours

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Principle of the method

The human IL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Target information

Interleukin-2 (IL-2), also known as T Cell Growth Factor, is a 15.5 kDa glycoprotein secreted primarily by activated T lymphocytes. The protein consists of 133 amino acids and is the product of several post-translational processing steps. Transcription and release of synthesized IL-2 is stimulated by antigen or mitogen activation of mature T lymphocytes. IL-2 binds to cell surface receptors and promotes the clonal expansion of antigen-specific effector T cells. IL-2 is a T cell differentiation factor, able to induce the production of other lymphokines, such as IL-4 and interferon-γ. IL-2 promotes the growth of B cells and induction of immunoglobulin secretion. IL-2 stimulates J chain synthesis which leads to assembly and secretion of IgM. Myeloid cell populations such as macrophage precursors and primary peripheral blood monocytes express IL-2 receptors. The binding of IL-2 will cause, in these cells, proliferation, differentiation and enhancement of cytolytic activity.*

* Please refer to ELISA protocol for specific reference citations.

Related links

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Learn more about other immunoassays

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: Cytokines & Receptors
Assay Incubation Time: 3 hr
Assay Range: 15.6-1000 pg/mL
Assay Sensitivity: <8 pg/mL
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Novex
Gene Aliases: IL-2
Gene ID: 16183
Gene Name: interleukin 2
Gene Symbol: IL2
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 5 x 96 tests
Protein Aliases: IL-2, T-cell growth factor, TCGF
Protein Name: Interleukin-2
Sample Type (Specific): Cell Culture Supernatants, Plasma, Serum
Sample Volume: 50 µL
Shipping Condition: Wet Ice
Target (Kit Named): IL-2
Target Species (Named): Mouse
Target Species (Validated): Mouse
UniProt ID: P04351

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols