IL-6 ELISA Kit, Mouse

Catalog number: KMC0061

Novex™  Related applications: Protein Assays and Analysis

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Description

The Mouse Interleukin-6 (IL-6, IL6) ELISA research-use-only kit is to be used for the quantitative determination of IL-6 in samples (see sample types indicated) using 96-well plates and a microplate reader. The assay will recognize both natural and recombinant forms of this target.

Performance characteristics

• Sensitivity: <3 pg/mL
• Standard curve range: 7.8–500 pg/mL
• Sample type(s): serum, plasma, cell culture supernatant
• Specificity: natural and recombinant human IL-6
• Cross-reactivity: see kit manual for cross-species and/or cross-target reactivity
• Sample volume: 50 μL, 100 μL (TCS)
• Total assay time: 3.5 hours

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Principle of the method

The mouse IL-6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Target information

IL-6 is a 21–28 kDa glycoprotein composed of 184 amino acids produced by lymphocytes, monocytes, fibroblasts, keratinocytes, endothelial cells, mesangial cells, astrocytes, bone marrow stroma, and tumor cells. At the protein level, mouse IL-6 shows 42% homology to human IL-6, while mouse and rat are 93% identical. IL-6 plays a major role in the regulation of cell growth, hematopoiesis, and inflammation. IL-6 induces maturation of B cells into antibody-secreting plasma cells and it co-stimulates T cell growth and cytotoxic T cell differentiation. IL-6 increases IL-2 receptor production in T cells and induces production of IL-2. IL-6 is the major inducer of acute phase reactions in response to inflammation or tissue injury, and with IL-1β and TNF-α, IL-6 induces synthesis of acute phase proteins by hepatocytes.

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For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gene Aliases: Il-6
Accession Number: NM_031168.1, NP_112445.1
Shipping Condition: Wet Ice
Marker: IL-6
Gene ID: 16193
Species: Mouse
Gene Symbol: Il6
Sensitivity: <3 pg/mL
Target Gene: interleukin 6
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 assays
Concentration: 7.8-500 pg⁄ml
Sample Volume: 50 µl, 100 µl supernatant
Protein Family: Cytokines & Receptors
Incubation Time: 3.5 hrs
Detection Method: Colorimetric
Research Category: Immunology
Sample Type (Specific): Plasma, Serum, Supernatants
For Use With (Equipment): Microplate Reader

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols

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