TNF alpha Mouse ELISA Kit

Catalog number:  KMC3012

Invitrogen™  Related applications: Protein Assays and Analysis

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The Mouse Tumor Necrosis Factor alpha (TNF alpha, TNF) ELISA research-use-only kit is to be used for the quantitative determination of TNF alpha in samples (see sample types indicated) using 96-well plates and a microplate reader. The assay will recognize both natural and recombinant forms of this target.

Performance characteristics

• Sensitivity: <3 pg/mL
• Standard curve range: 15.6‒1,000 pg/mL
• Sample type(s): serum, plasma, cell culture supernatant
• Specificity: natural and recombinant mouse TNF alpha
• Cross-reactivity: see kit manual for cross-species and/or cross-target reactivity
• Sample volume: 50 μL
• Total assay time: 2.5 hours

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Principle of the method

The mouse TNF alpha solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Target information

Mouse TNF-α, also known as cachectin, is a glycoprotein composed of 156 amino acids produced mainly by monocytes and activated macrophages. TNF-α has been shown to possess tumoricidal activity as well as a variety of physiologic effects with most major organ systems. In the central nervous system, TNF-α is involved in fever, anorexia, and alterations in pituitary hormone release. In the cardiovascular system, TNF-α plays a role in shock, acute respiratory distress, and capillary leakage syndrome (procoagulation). TNF-α is instrumental in the process of acute tubular necrosis and nephritis in the kidney and ischemia, colitis, and hepatic necrosis in the gastrointestinal system. Major TNF-α effects on metabolism include LPL suppression, protein catabolism, lipid catabolism, ACTH release, and insulin resistance. TNF-α is defined by its participation in inflammatory activities, cell toxicity, increased NK function, and mediation of TNF-α tumor toxicity.

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For Research Use Only. Not for use in diagnostic procedures.


Protein Family: Cytokines & Receptors
Assay Incubation Time: 2.5 hr
Assay Range: 19.5-1250 pg/mL
Assay Sensitivity: <3 pg/mL
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Novex
Gene Aliases: DIF, TNF-a, TNF-alpha, TNFSF2, TNFalphaa, Tnfsf1a, Tnlg1f, Tnf
Gene ID: 21926
Gene Name: tumor necrosis factor
Gene Symbol: TNF
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 2 x 96 tests
Protein Name: tumor necrosis factor
Sample Type (Specific): Cell Culture Supernatants, Plasma, Serum
Sample Volume: 50 µL
Shipping Condition: Wet Ice
Target (Kit Named): TNF alpha
Target Species (Named): Mouse
Target Species (Validated): Mouse
UniProt ID: P06804

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols

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