This product will be discontinued by May 1, 2016. We recommend you switch to TA Cloning® Kit, with pCR™2.1 Vector and One Shot® INVαF' Chemically Competent E. coli.

TA Cloning® Kit, with pCR™2.1 Vector and One Shot® INVαF' Chemically Competent E. coli, no manual

Catalog number: KNM200001

Invitrogen™  Related applications: PCR Cloning

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Description

TA Cloning® Kits are designed for cloning Taq-amplified PCR products directly from a PCR reaction using an overnight ligation step. The kits use a pCR™ vector and yield ≥80% recombinants with ≥90% of the recombinants containing inserts.

Some of the convenient features of the pCR™ vectors are:

• 3´-T overhangs for direct ligation of Taq-amplified PCR products
• Choice of T7 (pCR™2.1) or T7 and Sp6 (pCR™II) promoters for in vitro RNA transcription and sequencing
• Versatile polylinker with flanking EcoR I sites for easy excision of inserts
• Choice of kanamycin or ampicillin selection
• Easy blue/white colony screening
• M13 forward and reverse primer sites for sequencing
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Vector Type: TA Cloning Vectors, pCR
Cloning Method: TA Cloning®
Bacterial or Yeast Strain: INVαF´
Product Size: 20 reactions

Contents & storage

TA Cloning® Kits contain linearized pCR™2.1 or pCR™II vector, T4 DNA ligase, 10X ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Competent cell kits contain One Shot® Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot® E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols