Use ProLong® Live Antifade Reagent for brightest fluorescent images with live cells

Filmtracer™ LIVE/DEAD® Biofilm Viability Kit

Catalog number: L10316

Molecular Probes™  Related applications: Microbiological Analysis

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Description

The FilmTracer™ LIVE⁄DEAD Biofilm Viability kit provides a two-color fluorescence assay of bacterial viability, based on membrane integrity, that has proven usefule for a diverse array of bacterial genera including those growing in biofilm communities. The LIVE⁄DEAD Biofilm Viability kit utilizes mixtures of our SYTO® 9 green fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide. These stains differ both in their spectral characteristics and in their ability to penetrate healthy bacterial cells. When used alone, the SYTO® 9 stain generally labels all bacteria in a population—those with intact membranes and those with damaged membranes. In contrast, propidium iodide penetrates only bacteria with damaged membranes, causing a rduction in the SYTO® 9 stain fluorescence with both dyes are present. Thus, with an appropriate mixture of the SYTO™ 9 and propdium iodide stains, bacteria with intact cell membranes stain fluorescent green, whereas bacteria with damaged membranes stain fluorescent red.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Liquid
Color: Green, Red
Label or Dye: SYTO® 9, Propidium iodide
Product Size: 1 kit
Emission Class: Visible
Detection Method: Fluorescent
Excitation Class: Visible
For Use With (Equipment): Microplate Reader, Fluorescence Microscope, Flow Cytometer, Confocal Microscope, Fluorescent Imager
Sub-Cellular Localization: Cytoplasm & Cytosol
Flow Cytometer Laser Lines: 488
Excitation⁄Emission (nm): 480⁄500, 635
Shipping Condition: Room Temperature

Contents & storage

2 ingredients, store in freezer (-5°C to -30°C); protect from light

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