AKT Pathway [phospho] Multispecies 7-Plex Panel for Luminex™ Platform

Catalog number: LHO0001

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

The AKT Pathway Phospho 7-Plex Panel for the Luminex™ platform is specifically designed for quantifying the phosphorylated proteins IR[pYpY1162/1163], IGF-1R[pYpY1135/1136], IRS-1[pS312], Akt[pS473], PRAS40[pT246], p70S6K[pTpS421/424], and GSK-3β[pS9] in cell lysates and tissue homogenates (Figure 1). The antibodies used in this assay are human, mouse and rat cross-reactive. This kit has not been tested for multiplexing with other markers. Should user elect to multiplex this kit with other Luminex kits, the assay conditions should be determined empirically for each specific application. By measuring 7 analytes simultaneously, the Luminex™ assay panel helps provide more data from each sample, saving both money and time compared to more traditional systems (such as ELISA) used in research. This assay panel is suitable for use with the Luminex™ 100™/200™ and FLEXMAP 3D™ systems.

Superior performance—accurate, reproducible, and sensitive quantitation of multiple proteins
High quality—fully qualified antibodies permit excellent specificity and sensitivity
Fast and easy protocols—perform your multiplex assay and analyze your data typically in less than one day

Select Signaling Analyte Panel
The AKT Pathway Phospho 7-Plex Panel for the Luminex™ platform provides reagents for the accurate, reproducible, and sensitive quantitation of proteins including:

Signaling Proteins
IR[pYpY1162/1163]
IGF-1R[pYpY1135/1136]
IRS-1[pS312]
Akt[pS473]
PRAS40[pT246]
p70S6K[pTpS421/424]
GSK-3β[pS9]

Luminex™ xMAP™ Technology-Effective and Proven Analytical Tool
The AKT Pathway Phospho 7-Plex Panel is based on xMAP™ technology. The use of a suspension bead-based technology enables the multiplexing capabilities of the Luminex™ assays. Polystyrene microspheres are internally dyed with red and infrared fluorophores of differing intensities. Each bead is given a unique number, or bead region, allowing differentiation of one bead from another. Beads covalently bound to different antibodies can be mixed in the same assay, utilizing a 96-well microplate format. Upon completion of the sandwich immunoassay, beads must be measured using a Luminex™ detection system (100™/200™ or FLEXMAP 3D™). The instrument uses xPONENT™ software to distinguish bead color (analyte) and R-PE fluorescence intensity (assay signal strength) to quantify target(s).

More Validation for Confidence in Your Results
The AKT Pathway Phospho 7-Plex Panel has been validated using the same rigorous criterion that distinguishes Luminex™ assays from other commercially available assays. With more than 20 years of experience, our fully qualified antibodies help ensure the specificity and sensitivity of our assays. When benchmarked against the competition, our multiplex products consistently deliver excellent results. Every singleplex kit and premixed multiplex kit comes with a product insert that outlines the specifications for each marker in the assay (see Documents section below). Standard curves (Figure 2) are generated for each lot of product to assure quantitation consistency.

Learn more about all Invitrogen™ assays for the Luminex™ platform.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Marker: GSK-3β [pS9], IRS-1 [pS312], IR [pYpY1162⁄1163], IGF-1R [pYpY1135⁄1136], Akt [pS473], PRAS40 [pT246], p70S6K [pTpS421⁄ 424]
System: Luminex®
Gene ID: 67605 24185, 84027, 25718, 24954, 25467, 83840, 292887, 207, 2932, 3480, 3643, 3667, 6198, 84335, 11651, 56637, 16001, 16337, 16367, 72508
Species: Mouse, Rat, Human
Bead Type: Polystyrene
Bead Region: See Product Insert
Gene Symbol: AKT1, Gsk3b, Rps6kb1, Akt1s1, Akt1, RPS6KB1, INSR, IGF1R, IRS1, GSK3B, Igf1r, Irs1, Insr, AKT1S1
Sensitivity: See Product Insert
Label or Dye: R-PE
Product Size: 100 tests
Protein Family: Kinases & Inhibitors
Protein Subtype: Serine⁄Threonine Kinases
Detection Method: Fluorescent
Research Category: Signal Transduction
Sample Type (Specific): Cell Extracts, Tissue Homogenates
For Use With (Equipment): Luminex® Instruments
Shipping Condition: Wet Ice

Contents & storage

Contains premixed antibody coated capture beads, standard, premixed detector antibody, diluents, SAV concentrate, buffers, wash solution, filter plate, complete protocol and lot-specific technical data sheet. Store at 2-8°C.

Documents

Manuals & protocols

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