AmpliTaq® DNA Polymerase, LD (Low DNA) with Buffer II

Catalog number: N8080158

Applied Biosystems™  Related applications: PCR

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Description

Applied Biosystems® AmpliTaq® DNA Polymerase, LD (Low DNA), is the same enzyme as AmpliTaq® DNA Polymerase, but is further purified through a proprietary separation process to ensure that residual bacterial DNA sequences are substantially reduced.

Manufacturing process results in highly purified enzyme with lower levels of bacterial DNA

• Minimizes non-specific PCR products when amplifying bacterial targets
• Highly purified enzyme making it particularly useful for low copy number amplifications

High-Quality
This highly purified enzyme preparation ensures that non-target, false-positive PCR products will be effectively minimized when amplifying bacterial sequences. This is especially useful for low-copy-number PCR amplifications. The enzyme is quality-control tested to verify that fewer than 10 copies of bacterial 16S ribosomal RNA gene sequences are present in a standard 2.5 unit aliquot. AmpliTaq® DNA Polymerase, LD is supplied with GeneAmp® 10X PCR Buffer II and MgCl2 Solution. It is also available with GeneAmp® 10X PCR Buffer I.

Note:
See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: AmpliTaq® DNA Polymerase
Hot Start: No
Fidelity (vs. Taq): 1 X
Reaction Format: Separate Components
Product Overhang: 3'-A
Reaction Speed: Standard
Final Product Size(s): 5 kb or less
Product Size: 250 units
Exonuclease Activity: 5' - 3'
Purity or Quality Grade: LD (Low DNA)
Shipping Condition: Dry Ice

Contents & storage

Contains 250 units of AmpliTaq® DNA Polymerase, LD, Buffer II, and MgCl2 Solution. Store at –15 to –30°C.

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Manuals & protocols

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