AmpliTaq® DNA Polymerase with Buffer I (250 units/tube)

Catalog number: N8080160

Applied Biosystems™  Related applications: PCR

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

AmpliTaq® DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot.

Features of this enzyme:
  • AmpliTaq® DNA Polymerase is the most thoroughly characterized enzyme available for PCR, a testimony to its overall utility and efficacy
  • Its thermal activity profile makes it reliable for PCR applications
  • It is QC-tested to guarantee reproducible results

Reliable and robust PCR
The thermal activity profile of AmpliTaq® DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55°C–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. AmpliTaq® DNA Polymerase is supplied with GeneAmp® 10X PCR Buffer I. It is also available with GeneAmp® 10X PCR Buffer II and MgCl2 Solution.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: AmpliTaq® DNA Polymerase
Hot Start: No
Fidelity (vs. Taq): 1 X
Reaction Format: Separate Components
Product Overhang: 3'-A
Reaction Speed: Standard
Final Product Size(s): 5 kb or less
Product Size: 250 units
Exonuclease Activity: 5' - 3'
Shipping Condition: Dry Ice

Contents & storage

Contents:
  • 50 µL of AmpliTaq® DNA Polymerase (5 U/µL)
  • 1.5 mL of GeneAmp® 10X PCR Buffer I (100 mM Tris-HCl pH 8.3, 500 mM KCl, 15 mM MgCl2, 0.01% (w/v) gelatin).
Store at -20°C.

Documents

Manuals & protocols

Recommended products