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Oregon Green® 488 BAPTA-2, AM, cell permeant - Special Packaging

Catalog number: O6809

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Pharma & Biopharma | Target & Lead Identification & Validation

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Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+. They have uses in many calcium signaling investigations, including measuring intracellular Ca2+, following Ca2+ influx and release, and multiphoton excitation imaging of Ca2+ in living tissues. Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells. The fluorescence signal from these cells is generally measured using fluorescence microscopy, fluorescence microplate assays, or flow cytometry.

Calcium Indicator (AM Ester) Specifications:
• Label (Ex/Em): Oregon Green® 488 BAPTA-2 (494/523 nm)
• Fluorescence intensity increase upon binding Ca2+ : ~100 fold
• Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength

Spectral Characteristics of Molecular Probes® Calcium Indicators
These probes are excited by visible light, and because the energy required for excitation is low, the potential for cellular photodamage is reduced. Commonly used laser-based instruments (i.e., confocal laser scanning microscopes) are able to efficiently excite these indicators, and their emissions are in regions of the spectrum where cellular autofluorescence and scattering backgrounds are often less of a problem.

More Choices for Fluorescent Calcium Indicators
We offer a large selection of Molecular Probes® calcium indicators for use in various experimental scenarios, for example dextran versions for reduced leakage and compartmentalization and BAPTA conjugates for detecting high-amplitude calcium transients. For more information, review Fluorescent Ca2+ Indicators Excited with Visible Light—Section 19.3 in the Molecular Probes® Handbook.

For UV-excitable Ca2+ indicators, protein-based Ca2+ indicators, conjugates of Ca2+ indicators, and for fluorescence-based indicators of other metal ions (i.e., Mg2+, Zn2+) review Indicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions—Chapter 19 in the Molecular Probes® Handbook.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.


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Indicator Type: Calcium Indicator
Indicator Properties: Fluorescent Dye-Based
For Use With (Equipment): Microplate Reader, Flow Cytometer, Fluorescence Microscope
Cell Permeability: Cell-Permeant
Product Size: 10 x 50 µg
Detection Method: Fluorescent
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.


Manuals & protocols

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